Using wintergreen oil for mounting mosquito larvae: a safer alternative to xylene
| Autor: | F. M. Salleh, M. A G Nashithatul, J. B. Koay, R. Ihsanuddin, N. N. Natasya, Aishah Hani Azil |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Veterinary medicine animal structures Histology 030209 endocrinology & metabolism Biology Xylenes 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Mosquito larvae Aedes Oils Volatile Species identification Animals Clearing Agent Laboratory technicians Histocytological Preparation Techniques Plant Extracts fungi Xylene General Medicine Anatomy Salicylates Medical Laboratory Technology 030104 developmental biology chemistry Larva Instar |
| Zdroj: | Biotechnichistochemistry : official publication of the Biological Stain Commission. 91(1) |
| ISSN: | 1473-7760 |
| Popis: | Permanent mounting of fourth instar mosquito larvae is essential for identifying Aedes spp. This procedure requires extensive exposure to xylene, a clearing agent in the mounting process. We investigated wintergreen oil as a substitute for xylene. Five hundred larvae were mounted on slides to evaluate shrinkage or expansion of specimens after clearing using xylene or wintergreen oil. We examined the ventral brush and siphonal hair tufts for species identification and for preservation of morphological characteristics after clearing specimens in xylene or wintergreen oil. Shrinkage of the length of whole larvae and width of the head, thorax and abdomen after mounting was significantly greater after clearing with xylene than with wintergreen oil. The length of the comb scale nearest the ventral brush was similar for both clearing agents. The clarity of the specimens after mounting was improved by clearing with wintergreen oil, but the integrity of the ventral brush and siphonal hair tufts were similar for both clearing agents. |
| Databáze: | OpenAIRE |
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