Growth-hormone-releasing peptide 6 (GHRP6) prevents oxidant cytotoxicity and reduces myocardial necrosis in a model of acute myocardial infarction
| Autor: | Nelson Santiago Vispo, Gerardo Guillén, Danay Cibrian, Alberto Rodriguez, José Suárez Alba, Jorge Berlanga, Brumny Perez, Heberto Dominguez, Pedro Lopez-Saura, Luis Guevara, Diana García, Ernesto López-Mola, Alina Seralena |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Cardiotonic Agents Necrosis Cell Survival Swine Myocardial Infarction Ischemia Infarction medicine.disease_cause Antioxidants Electrocardiography Internal medicine Animals Creatine Kinase MB Form Medicine Myocytes Cardiac Myocardial infarction Insulin-Like Growth Factor I Cardioprotection business.industry Myocardium Arrhythmias Cardiac Organ Size General Medicine medicine.disease Growth hormone secretion Disease Models Animal Oxidative Stress C-Reactive Protein Endocrinology Coronary occlusion Female medicine.symptom business Oligopeptides Oxidation-Reduction Oxidative stress |
| Zdroj: | Clinical Science. 112:241-250 |
| ISSN: | 1470-8736 0143-5221 |
| DOI: | 10.1042/cs20060103 |
| Popis: | Therapies aimed at enhancing cardiomyocyte survival following myocardial injury are urgently required. As GHRP6 [GH (growth hormone)-releasing peptide 6] has been shown to stimulate GH secretion and has beneficial cardiovascular effects, the aim of the present study was to determine whether GHRP6 administration reduces myocardial infarct size following acute coronary occlusion in vivo. Female Cuban Creole pigs were anaesthetized, monitored and instrumented to ensure a complete sudden left circumflex artery occlusion for 1 h, followed by a 72 h reperfusion/survival period. Animals were screened clinically before surgery and assigned randomly to receive either GHRP6 (400 μg/kg of body weight) or normal saline. Hearts were processed, and the area at risk and the infarct size were determined. CK-MB (creatine kinase MB) and CRP (C-reactive protein) levels and pathological Q-wave-affected leads were analysed and compared. Evaluation of the myocardial effect of GHRP6 also included quantitative histopathology, local IGF-I (insulin-growth factor-I) expression and oxidative stress markers. GHRP6 treatment did not have any influence on mortality during surgery associated with rhythm and conductance disturbances during ischaemia. Infarct mass and thickness were reduced by 78% and 50% respectively, by GHRP6 compared with saline (P |
| Databáze: | OpenAIRE |
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