Phenotypic characteristics and population genetics of Enterococcus faecalis cultured from patients in Tehran during 2000-2001
| Autor: | Ahmad Asgharzadeh, Fatemeh Mobasheri, Soroor Asadi, Gelavizh Etemadi, Mohammad Mehdi Feizabadi, Atusa Aliahmadi |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Electrophoresis
Imipenem Immunology Population Drug resistance Microbial Sensitivity Tests Iran Applied Microbiology and Biotechnology Microbiology Enterococcus faecalis Ampicillin Drug Resistance Multiple Bacterial Genetics medicine Humans education Molecular Biology Gram-Positive Bacterial Infections education.field_of_study Cross Infection biology Genetic Variation General Medicine biology.organism_classification Anti-Bacterial Agents Bacterial Typing Techniques Culture Media Enzymes Ciprofloxacin Penicillin Hospitalization Phenotype Gentamicin medicine.drug |
| Zdroj: | Canadian journal of microbiology. 49(10) |
| ISSN: | 0008-4166 |
| Popis: | Conventional bacteriology techniques were used to identify enterococci isolates cultured from patients at different hospitals in Tehran during 2000–2001. The identification was confirmed using species-specific PCR targeting the D-alanyl-D-alanine ligase gene. A total of 59 isolates of Enterococcus faecalis were identified. The rates of resistance to different antibiotics were in the following order: penicillin 84%, ciprofloxacin 42%, high-level gentamicin 30%, nitrofurantoin 14%, imipenem 4%, and chloramphenicol 2%. Resistance to ampicillin was found to be rare among the Iranian isolates of E. faecalis. Multi-locus enzyme electrophoresis was then used to analyze the strains. Forty-five electrophoretic types were obtained when 10 enzyme loci were screened. Although the collection of bacterial isolates was limited in time and location, considerable heterogeneity was found. Analysis of strains for linkage disequilibrium demonstrated that the studied population is not clonal, since the index of association was not significantly different from zero (Ia= 0.0296). Enterococcus faecalis isolates recovered from patients in Tehran were genetically diverse and seemed to possess a high potential for genetic recombinations, though none were resistant to vancomycin.Key words: Enterococcus faecalis, population genetics, MEE analysis, nosocomial infections. |
| Databáze: | OpenAIRE |
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