Detection of alpha1 integrin in urine of patients with immunoglobulin A nephropathy
| Autor: | Ram Doolman, Jonathan Bank, Aharon Ben-David, Ben-Ami Sela, Ilan Bank |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Biopsy Integrin Integrin alpha1 Enzyme-Linked Immunosorbent Assay Urine General Biochemistry Genetics and Molecular Biology medicine Humans Secretion Immunoglobulin A Nephropathy Aged Aged 80 and over Kidney biology medicine.diagnostic_test business.industry Glomerulonephritis IGA General Medicine Middle Aged medicine.disease medicine.anatomical_structure Immunology biology.protein Female business Nephritis Kidney disease |
| Zdroj: | Journal of investigative medicine : the official publication of the American Federation for Clinical Research. 56(3) |
| ISSN: | 1081-5589 |
| Popis: | Background The α1β1 integrin is a cell surface membrane heterodimer composed of noncovalently linked α1 and β1 polypeptides that is up-regulated on activated and proliferating mesangial cells. Methods A double-sandwich enzyme-linked immunosorbent assay that detects α1 integrin in a specific and dose-dependent manner at concentrations greater than 150 ng/mL was used to evaluate whether intact α1 polypeptides are secreted in the urine samples of 29 patients with various kidney diseases and in those of 5 healthy individuals. Results α1 Integrin was detected in 8 of the 29 patients including 3 of 3 patients with biopsy-proven immunoglobulin A nephropathy and 3 of 3 clinically suspected but non-biopsy-proven immunoglobulin A nephropathy with evidence of active nephritis. No α1 integrins were found in samples of 5 healthy controls. Conclusions α1 Integrin polypeptides can be detected in human urine, particularly in immunoglobulin A nephropathy. Further extensive studies are required to clarify the significance of secretion of α1 integrins in urine of patients with kidney disease. |
| Databáze: | OpenAIRE |
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