In vitro differentiation of human multilineage differentiating stress-enduring (Muse) cells into insulin producing cells
| Autor: | Sherry M. Khater, Mahmoud M. Zakaria, Ali M. Fouad, Mahmoud M. Gabr, Ayman F. Refaie, Amani M. Ismail, Elsayed K. Abdelhady |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:QH426-470 medicine.medical_treatment lcsh:Biotechnology Medical Biotechnology Muse cells Fluorescence activated cell sorting Biology law.invention 03 medical and health sciences Fluorescence-Activated Cell Sorting Cell sorter Confocal microscopy law lcsh:TP248.13-248.65 medicine General Materials Science Real time-quantitative PCR Insulin Mesenchymal stem cell Embryonic stem cell In vitro Cell biology Heterogeneous population lcsh:Genetics 030104 developmental biology Adipose derived mesenchymal stem cells Mesenchymal stem cells |
| Zdroj: | Journal of Genetic Engineering and Biotechnology, Vol 16, Iss 2, Pp 433-440 (2018) Journal of Genetic Engineering & Biotechnology |
| Popis: | Mesenchymal stem cells (MSCs) is a heterogeneous population. Muse cells is a rare pluripotent subpopulation within MSCs. This study aims to evaluate the pulirpotency and the ability of Muse cells to generate insulin producing cells (IPCs) after in vitro differentiation protocol compared to the non-Muse cells. Muse cells were isolated by FACSAria III cell sorter from adipose-derived MSCs and were evaluated for its pluripotency. Following in vitro differentiation, IPCs derived from Muse and non-Muse cells were evaluated for insulin production. Muse cells comprised 3.2 ± 0.7% of MSCs, approximately 82% of Muse cells were positive for anti stage-specific embryonic antigen-3 (SSEA-3). Pluripotent markers were highly expressed in Muse versus non-Muse cells. The percentage of generated IPCs by flow cytometric analysis was higher in Muse cells. Under confocal microscopy, Muse cells expressed insulin and c-peptide while it was undetected in non-Muse cells. Our results introduced Muse cells as a new adult pluripotent subpopulation, which is capable to produce higher number of functional IPCs. Keywords: Muse cells, Adipose derived mesenchymal stem cells, Fluorescence activated cell sorting, Real time-quantitative PCR, Mesenchymal stem cells |
| Databáze: | OpenAIRE |
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