Cloning of the mucosal addressin MAdCAM-1 from human brain: Identification of novel alternatively spliced transcripts
| Autor: | Ries J. Langley, Euphemia Leung, Klaus Lehnert, Jian Ni, L G Raymond, John M. Greene, G. W. Krissansen |
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| Rok vydání: | 1996 |
| Předmět: |
0301 basic medicine
Signal peptide Transcription Genetic Molecular Sequence Data Immunology Receptors Lymphocyte Homing Immunoglobulins Immunoglobulin domain Mice 03 medical and health sciences Mucoproteins 0302 clinical medicine Addressin Animals Humans Immunology and Allergy Amino Acid Sequence RNA Messenger Cloning Molecular Peptide sequence Brain Chemistry chemistry.chemical_classification Expressed sequence tag Mucous Membrane Base Sequence biology Chemistry cDNA library Membrane Proteins Cell Biology Molecular biology Amino acid Alternative Splicing Transmembrane domain 030104 developmental biology Antigens Surface biology.protein Cell Adhesion Molecules 030215 immunology |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0818-9641 |
| Popis: | The mucosal addressin cell adhesion molecule-1 (MAdCAM-1), expressed selectively on high endothelial venules (HEV) and lamina propria venules, directs lymphocyte traffic by binding the lymphocyte Peyer's patch adhesion molecule-1 (LPAM-1, alpha 4 beta 7). Full-length DNA encoding human MAdCAM-1 was obtained by combining sequences from an expressed sequence tag (EST) identified in an early stage human brain cDNA library, a polymerase chain reaction-derived clone, and a MAdCAM-1 genomic clone. The deduced amino acid sequence revealed an 18 amino acid signal peptide, two N-terminal immunoglobulin (Ig)-like domains conserved (59-65%) in sequence with those of the mouse homologue, an 86 amino acid mucin-like region rich in serine-threonine residues, a 20 amino acid transmembrane domain and a 43 amino acid charged cytoplasmic domain. No counterpart to the third IgA-like domain of mouse MAdCAM-1 was present; however, the serine-threonine-rich mucin domain was extended as two distinguishable major and minor mucin regions unrelated to the mouse domain. The major domain is formed from six tandem repeats of an eight amino acid sequence having the MUC-2-related consensus DTTSPEP/SP. Human MAdCAM-1 mRNA transcripts were restricted to small intestine, colon, spleen, pancreas and brain. Alternatively spliced MAdCAM-1 variants were identified that lack parts of the second Ig domain and all or part of the major mucin domain, indicating that the function of this vascular addressin is regulated by extensive modifications to its multi-domain structure. |
| Databáze: | OpenAIRE |
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