Mangiferin Accelerates Glycolysis and Enhances Mitochondrial Bioenergetics

Autor: Yuling Chi, Ekaterina Vladimirovna Fomenko, Irwin J. Kurland, Victor L. Schuster, Jeffrey E. Pessin, Pasha Apontes, Nan Chi, Zhongbo Liu
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Bioenergetics
Mitochondrion
lcsh:Chemistry
Mice
transcriptomics
0302 clinical medicine
Glycolysis
glucose
lcsh:QH301-705.5
Spectroscopy
Glyceraldehyde 3-phosphate dehydrogenase
biology
Chemistry
Succinate dehydrogenase
Glyceraldehyde-3-Phosphate Dehydrogenases
General Medicine
glycolysis
metabolomics
Mitochondria
Computer Science Applications
Cell biology
Succinate Dehydrogenase
Metabolome
Xanthones
Citric Acid Cycle
Carbohydrate metabolism
Diet
High-Fat
DNA
Mitochondrial
Article
Catalysis
mangiferin
Cell Line
mitochondrial bioenergetics
Inorganic Chemistry
03 medical and health sciences
Animals
Physical and Theoretical Chemistry
Molecular Biology
TCA cycle
Organic Chemistry
Mice
Inbred C57BL
Citric acid cycle
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
biology.protein
Energy Metabolism
Flux (metabolism)
030217 neurology & neurosurgery
Zdroj: International Journal of Molecular Sciences; Volume 19; Issue 1; Pages: 201
International Journal of Molecular Sciences, Vol 19, Iss 1, p 201 (2018)
International Journal of Molecular Sciences
ISSN: 1422-0067
DOI: 10.3390/ijms19010201
Popis: One of the main causes of hyperglycemia is inefficient or impaired glucose utilization by skeletal muscle, which can be exacerbated by chronic high caloric intake. Previously, we identified a natural compound, mangiferin (MGF) that improved glucose utilization in high fat diet (HFD)-induced insulin resistant mice. To further identify the molecular mechanisms of MGF action on glucose metabolism, we conducted targeted metabolomics and transcriptomics studies of glycolyic and mitochondrial bioenergetics pathways in skeletal muscle. These data revealed that MGF increased glycolytic metabolites that were further augmented as glycolysis proceeded from the early to the late steps. Consistent with an MGF-stimulation of glycolytic flux there was a concomitant increase in the expression of enzymes catalyzing glycolysis. MGF also increased important metabolites in the tricarboxylic acid (TCA) cycle, such as α-ketoglutarate and fumarate. Interestingly however, there was a reduction in succinate, a metabolite that also feeds into the electron transport chain to produce energy. MGF increased succinate clearance by enhancing the expression and activity of succinate dehydrogenase, leading to increased ATP production. At the transcriptional level, MGF induced mRNAs of mitochondrial genes and their transcriptional factors. Together, these data suggest that MGF upregulates mitochondrial oxidative capacity that likely drives the acceleration of glycolysis flux.
Databáze: OpenAIRE
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