Metabolic and physiological interdependencies in the Bathymodiolus azoricus symbiosis
| Autor: | Stephanie Markert, Nicole Dubilier, Doerte Becher, Martin Moche, Andreas Otto, Thomas Schweder, Manuel Kleiner, Noriyuki Satoh, Lizbeth Sayavedra, Jillian M. Petersen, Takeshi Takeuchi, Ruby Ponnudurai |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Gills
0301 basic medicine animal structures Proteome Microorganism Sulfur metabolism Biology Microbiology 03 medical and health sciences Hydrothermal Vents Species Specificity Microbial ecology Symbiosis Gammaproteobacteria Animals Ecology Evolution Behavior and Systematics Host (biology) fungi food and beverages biochemical phenomena metabolism and nutrition biology.organism_classification Biosynthetic Pathways 030104 developmental biology Calyptogena magnifica Environmental biotechnology Biochemistry Mytilidae bacteria Original Article Methane Oxidation-Reduction Sulfur |
| Zdroj: | ISME JOURNAL The ISME Journal |
| Popis: | The hydrothermal vent mussel Bathymodiolus azoricus lives in an intimate symbiosis with two types of chemosynthetic Gammaproteobacteria in its gills: a sulfur oxidizer and a methane oxidizer. Despite numerous investigations over the last decades, the degree of interdependence between the three symbiotic partners, their individual metabolic contributions, as well as the mechanism of carbon transfer from the symbionts to the host are poorly understood. We used a combination of proteomics and genomics to investigate the physiology and metabolism of the individual symbiotic partners. Our study revealed that key metabolic functions are most likely accomplished jointly by B. azoricus and its symbionts: (1) CO2 is pre-concentrated by the host for carbon fixation by the sulfur-oxidizing symbiont, and (2) the host replenishes essential biosynthetic TCA cycle intermediates for the sulfur-oxidizing symbiont. In return (3), the sulfur oxidizer may compensate for the host's putative deficiency in amino acid and cofactor biosynthesis. We also identified numerous ‘symbiosis-specific' host proteins by comparing symbiont-containing and symbiont-free host tissues and symbiont fractions. These proteins included a large complement of host digestive enzymes in the gill that are likely involved in symbiont digestion and carbon transfer from the symbionts to the host. |
| Databáze: | OpenAIRE |
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