The induction of aryl hydrocarbon receptor (AHR) in immune organs of developing chicks by polycyclic aromatic hydrocarbons

Autor:	H. Hazilawati, A. R. Nisha, Mohamed Mustapha Noordin, M. L. Mohd Azmi
Rok vydání:	2018
Předmět:	0301 basic medicine animal structures Health Toxicology and Mutagenesis Embryonic Development Spleen Chick Embryo Thymus Gland Toxicology 03 medical and health sciences chemistry.chemical_compound Bursa of Fabricius 0302 clinical medicine medicine Animals Polycyclic Aromatic Hydrocarbons Fluoranthene Dose-Response Relationship Drug biology Phenanthrene Aryl hydrocarbon receptor Molecular biology Reverse transcription polymerase chain reaction 030104 developmental biology medicine.anatomical_structure Receptors Aryl Hydrocarbon chemistry Organ Specificity biology.protein Pyrene Environmental Pollutants Xenobiotic 030217 neurology & neurosurgery
Zdroj:	Toxicology Mechanisms and Methods. 28:461-466
ISSN:	1537-6524 1537-6516
DOI:	10.1080/15376516.2018.1459992
Popis:	Polycyclic aromatic hydrocarbons are pollutants which are persistent in nature. The aryl hydrocarbon receptor is a ligand-activated cytosolic transcription factor activated by xenobiotics. The objective was to isolate and identify AHR mRNA transcript in immune organs of developing chicks and to interpret the correlation between AHR induction and dose of PAHs. Specific pathogen free embryonated eggs on day nine were inoculated with solutions of pyrene, phenanthrene, and fluoranthene dissolved in tricaprylin (vehicle) through the allantoic route at three dose levels: 0.2 mg/kg, 2 mg/kg, and 20 mg/kg. A 650 base pair product was observed by RNA extraction and reverse transcription PCR from thymus, bursa of Fabricius and spleen on 21st day. When AHR concentration was analyzed by ELISA in these organs, pyrene showed maximum potency in inducing AHR in thymus. Fluoranthene made highest concentration of AHR in bursa of Fabricius. None of these chemicals caused an increase in AHR concentration in spleen.
Databáze:	OpenAIRE
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