Regional expression of the BCRP/ABCG2 transporter in term human placentas
| Autor: | Christopher J. Gibson, Faith E. Archer, Lauren M. Aleksunes, Kristin M. Bircsak, Pamela Ohman-Strickland, Anna M. Vetrano, Naureen Memon, Mana M. Parast, Barry Weinberger |
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| Rok vydání: | 2014 |
| Předmět: |
Member 2
Adult medicine.medical_specialty Abcg2 ATP Binding Cassette Transporter ABCG2 Placenta Messenger Subfamily G Transporter Toxicology Article Paediatrics and Reproductive Medicine Clinical Research Pregnancy Internal medicine Complementary DNA Genetics medicine ATP Binding Cassette Transporter Subfamily G Member 2 Humans RNA Messenger Housekeeping gene Glyceraldehyde 3-phosphate dehydrogenase Messenger RNA Fetus biology Pharmacology and Pharmaceutical Sciences Neoplasm Proteins Endocrinology medicine.anatomical_structure embryonic structures Public Health and Health Services biology.protein RNA BCRP ATP-Binding Cassette Transporters Female |
| Zdroj: | Reproductive Toxicology. 43:72-77 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2013.11.003 |
| Popis: | The breast cancer resistance protein (BCRP, ABCG2) is an efflux transporter that removes xenobiotics that cross the placenta back to the maternal circulation, thereby limiting exposure of the fetus to drugs and chemicals. Currently, variability of BCRP expression within the placenta is not known. Ten placentas were collected from healthy women undergoing elective Cesarean sections at term. Villous samples were dissected in defined regions (medial, intermediate, and peripheral) and BCRP mRNA and protein were quantified. There were no regional differences in mRNA expression of housekeeping genes (GAPDH, RPL13a, PRL, 18S). GAPDH had the lowest correlation with BCRP Ct values and was used for BCRP mRNA normalization. No differences in placental BCRP mRNA and protein were observed among the sample sites ( |
| Databáze: | OpenAIRE |
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