Differential Increases in the Expression of Intermediate Filament Proteins and Concomitant Morphological Changes of Transdifferentiating Rat Hepatic Stellate Cells Observed In Vitro
| Autor: | Taku Hebiguchi, Yoshihiro Mezaki, Mitsutaka Miura, Kiwamu Yoshikawa, Mayako Morii, Hiroaki Yoshino, Haruki Senoo, Noriko Yamaguchi, Katsuyuki Imai |
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| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty Histology Physiology desmin Vimentin macromolecular substances Biochemistry Pathology and Forensic Medicine vimentin Downregulation and upregulation medicine Intermediate filament hepatic stellate cell intermediate filament Glial fibrillary acidic protein biology Transdifferentiation Regular Article hemic and immune systems Cell Biology myofibroblast Cell biology biology.protein Hepatic stellate cell Desmin Myofibroblast |
| Zdroj: | Acta Histochemica et Cytochemica |
| ISSN: | 1347-5800 0044-5991 |
| DOI: | 10.1267/ahc.13007 |
| Popis: | The primary function of hepatic stellate cells (HSCs) is the storage of vitamin A. However, they are also responsible for liver fibrosis and are therapeutic targets for treatment of liver cirrhosis. Among the many molecular markers that define quiescent or activated states of HSCs, the characteristics of type III intermediate filaments are of particular interest. Whereas vimentin and desmin are upregulated in activated HSCs, glial fibrillary acidic protein is downregulated in activated HSCs. The functional differences between vimentin and desmin are poorly understood. By time-course quantifications of several molecular markers for HSC activation, we observed that the expression of vimentin preceded that of desmin during the transdifferentiation of HSCs. The immunoreactivity of vimentin in transdifferentiated HSCs was more intense in perinuclear regions compared to that of desmin. We propose that the delayed expression of desmin following the expression of vimentin and the peripheral localization of desmin compared to vimentin are both related to the more extended phenotype of transdifferentiating HSCs observed in vitro. |
| Databáze: | OpenAIRE |
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