Analyses of microRNAs and transcriptomes in the midgut ofSpodoptera liturafeeding onBrassica juncea
Autor: | Yongjie Cen, Binbin Qiu, Kang Ma, Xiaopeng Zou, Yiguang Lin, Qili Feng, Sichun Zheng |
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Rok vydání: | 2020 |
Předmět: |
Messenger RNA
biology Spodoptera litura Midgut RNA-Seq Spodoptera biology.organism_classification General Biochemistry Genetics and Molecular Biology Diet Gastrointestinal Tract Transcriptome MicroRNAs Real-time polymerase chain reaction Biochemistry Larva Insect Science microRNA Animals Herbivory Agronomy and Crop Science Gene Ecology Evolution Behavior and Systematics Mustard Plant |
Zdroj: | Insect Science. 28:533-547 |
ISSN: | 1744-7917 1672-9609 |
Popis: | Spodoptera litura is a destructive agricultural pest in tropical and subtropical areas. Understanding the molecular mechanisms of S. litura adaptation to its preferred host plants may help identify target genes useful for pest control. We used high-throughput sequencing to characterize the expression patterns of messenger RNAs (mRNAs) and microRNAs (miRNAs) in the midgut of S. litura fed on Brassica juncea for 6 h and 48 h. A total of 108 known and 134 novel miRNAs were identified, 29 miRNAs and 237 mRNAs were differentially expressed at 6 h of B. juncea feeding, 26 miRNAs and 433 mRNAs were differentially expressed at 48 h. For the mRNAs, the up-regulated genes were mostly enriched in detoxification enzymes (cytochrome P450, esterase, glutathione S-transferase, uridine diphosphate-glucuronosyl transferase), while the down-regulated genes were mostly enriched in proteinases and immune-related genes. Furthermore, most detoxification enzymes begin to up-regulate at 6 h, while most digestion and immune-related genes begin to up- or down-regulate at 48 h. Eighteen and 37 differently expressed transcription factors were identified at 6 h and 48 h, which may regulate the functional genes. We acquired 136 and 41 miRNA versus mRNA pairs at 6 h and 48 h, respectively. Some down-regulated and up-regulated miRNAs were predicted to target detoxification enzymes and proteinases, respectively. Real-time quantitative polymerase chain reaction of nine randomly selected miRNAs and 28 genes confirmed the results of RNA-seq. This analyses of miRNA and mRNA transcriptomes provides useful information about the molecular mechanisms of S. litura response to B. juncea. |
Databáze: | OpenAIRE |
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