Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture System
| Autor: | Markus Neubauer, Olga Kuten-Pella, Zsombor Lacza, Andrea De Luna, Stefan Nehrer, Karina Kramer |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Adult Male QH301-705.5 Cartilage disorder Cell Culture Techniques blood products Osteoarthritis 3D pellet culture hyperacute serum Microbiology Regenerative medicine Chondrocyte Andrology Extracellular matrix 03 medical and health sciences 0302 clinical medicine Chondrocytes Osteogenesis Platelet-Rich Fibrin Gene expression medicine Humans Regeneration Biology (General) cartilage Molecular Biology Cells Cultured Chemistry Platelet-Rich Plasma osteoarthritic chondrocytes Cartilage General Medicine Middle Aged medicine.disease Immunohistochemistry Extracellular Matrix 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation 030220 oncology & carcinogenesis Platelet-rich plasma Female Energy Metabolism Biomarkers |
| Zdroj: | Current Issues in Molecular Biology, Vol 43, Iss 48, Pp 665-675 (2021) Current Issues in Molecular Biology Volume 43 Issue 2 Pages 48-675 |
| ISSN: | 1467-3045 |
| Popis: | Intra-articular injection of different types of blood-derived products is gaining popularity and clinical importance in the treatment of degenerative cartilage disorders such as osteoarthritis. The regenerative potential of two types of platelet-rich plasma (PRP), prepared in the presence of EDTA (EPRP) and citrate (CPRP) and an alternative blood product-hyperacute serum (hypACT) was evaluated using a 3D osteoarthritic chondrocyte pellet model by assessing the metabolic cell activity, cartilage-related gene expression and extracellular matrix deposition within the pellets. Chondrocyte viability was determined by XTT assay and it revealed no significant difference in metabolic activity of OA chondrocyte pellets after supplementation with different blood products. Nevertheless, the selection of blood products influenced the cartilage-related genes expression, ECM morphology and the tissue quality of pellets. Both PRP types had a different biological effect depending upon concentration and even though CPRP is widely used in clinics our assessment did not reveal good results in gene expression either tissue quality. HypACT supplementation resulted in superior cartilage-related genes expression together with tissue quality and seemed to be the most stable product since no remarkable changes were observed between the two different concentrations. All in all, for successful regenerative therapy, possible molecular mechanisms induced by blood-derived products should be always carefully investigated and adapted to the specific medical indications. |
| Databáze: | OpenAIRE |
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