Usutu virus growth in human cell lines: induction of and sensitivity to type I and III interferons
| Autor: | Carolina Scagnolari, Alessandra della Torre, Lucia Spano, Annalisa Soldà, Norbert Nowotny, Giulia Cacciotti, Beniamino Caputo, Simona Trombetti, Paolo Villari, Guido Antonelli |
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| Rok vydání: | 2012 |
| Předmět: |
0303 health sciences
biology 030306 microbiology Human cell biology.organism_classification Virus Replication Viral infection Virology Replication cycle 3. Good health Cell Line Pathogenesis 03 medical and health sciences Interferon Encephalitis Viruses Japanese Chlorocebus aethiops Vero cell medicine Animals Humans Interferons Usutu virus 030304 developmental biology medicine.drug |
| Zdroj: | Journal of General Virology; Vol 94 |
| ISSN: | 1465-2099 |
| Popis: | The mechanisms of Usutu virus (USUV) pathogenesis are largely unknown. The aim of this study was to evaluate the sensitivity of USUV to interferon (IFN) and the capacity of USUV to stimulate IFN production. Initial experiments were conducted to characterize the susceptibility of human cell lines to USUV infection and to evaluate the single-growth cycle replication curve of USUV. Results indicate that USUV is able to infect a variety of human cell lines, completing the replication cycle in Hep-2 and Vero cells within 48 h. Pre-treatment of cells with types I and III IFNs significantly inhibited the replication of USUV. However, the inhibitory effects of IFNs were considerably less if IFN was added after viral infection had been initiated. Also, USUV weakly induced types I and III IFNs. |
| Databáze: | OpenAIRE |
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