Virgibacillus arcticus sp. nov., a moderately halophilic, endospore-forming bacterium from permafrost in the Canadian high Arctic
| Autor: | Sophie Charvet, Beatrice Barbier, Blaire Steven, Thomas D. Niederberger, Lyle G. Whyte |
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| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Canada Molecular Sequence Data Virgibacillus arcticus Peptidoglycan Sodium Chloride Permafrost DNA Ribosomal Microbiology Endospore chemistry.chemical_compound Cell Wall RNA Ribosomal 16S Optimum growth Cluster Analysis Bacillaceae Phylogeny Soil Microbiology Ecology Evolution Behavior and Systematics Spores Bacterial Base Composition biology Arctic Regions Ecology Fatty Acids Quinones Temperature Nucleic Acid Hybridization Sequence Analysis DNA General Medicine 16S ribosomal RNA biology.organism_classification Halophile Bacterial Typing Techniques chemistry Bacteria |
| Zdroj: | INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 59:2219-2225 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | A novel, moderately halophilic, endospore-forming bacterial strain, designated Hal 1T, was isolated from a permafrost core collected from the Canadian high Arctic. The temperature for growth of strain Hal 1T was 0-30 degrees C with no growth observed at either -5 or 37 degrees C (optimum growth at about 25 degrees C). Strain Hal 1T was able to grow at NaCl concentrations of 0-20% (w/v) and did not have an absolute NaCl requirement for growth; optimal growth was at 5% (w/v) NaCl. The level of 16S rRNA gene sequence similarity between strain Hal 1T and the type strains of Virgibacillus carmonensis and Virgibacillus necropolis was 98.2%; values with respect to the type strains of other recognized Virgibacillus species were below 96.0%. The DNA G+C content of strain Hal 1T was 38.2 mol%. Levels of DNA-DNA relatedness between strain Hal 1T and the type strains of V. carmonensis and V. necropolis were 14.0 and 21.0%, respectively. The major fatty acid of strain Hal 1T was anteiso-C15:0, consistent with species of the genus Virgibacillus. The cell-wall peptidoglycan of strain Hal 1T was type A1alpha and the major respiratory quinone was MK-7. On the basis of genotypic and physiological results, strain Hal 1T (=DSM 19574T=JCM 14839T) is proposed as the type strain of a novel species of the genus Virgibacillus, namely Virgibacillus arcticus sp. nov. |
| Databáze: | OpenAIRE |
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