The effect of follicullar fluid pesticides and polychlorinated biphenyls concentrations on intracytoplasmic sperm injection (ICSI) embryological and clinical outcome
| Autor: | Ihab Elnashar, Omar M. Shabaan, Tarek A. Farghaly, Rashad M. Mostafa, Tarek K. Al-Hussaini, Ahmed A. Abdelaleem, Sahar E M El-Deek, Mona A.H. El-Baz |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Diazinon Pregnancy Rate medicine.medical_treatment Allethrins 010501 environmental sciences 01 natural sciences Intracytoplasmic sperm injection DDT Andrology 03 medical and health sciences chemistry.chemical_compound Human fertilization Pregnancy Nitriles Pyrethrins medicine Humans Bioallethrin Sperm Injections Intracytoplasmic Pesticides 0105 earth and related environmental sciences business.industry Pregnancy Outcome Obstetrics and Gynecology Pesticide Polychlorinated Biphenyls Follicular fluid Follicular Fluid Pregnancy rate Cross-Sectional Studies 030104 developmental biology Reproductive Medicine chemistry Malathion Environmental Pollutants Female Chlorpyrifos Lindane business Hexachlorocyclohexane |
| Zdroj: | European Journal of Obstetrics & Gynecology and Reproductive Biology. 220:39-43 |
| ISSN: | 0301-2115 |
| Popis: | Objectives The present study evaluated the correlation between the concentrations of polychlorinated biphenyls (PCBs) and pesticides in the follicular fluid (FF) obtained during intracytoplasmic sperm injection (ICSI) with the ovarian response, endometrial thickness, and embryological and clinical outcomes. Design Cross-sectional observational study. Materials and methods Women aged 20 to 38 years (300 infertile couples) presenting to a university-affiliated fertility center were approached to participate in the study. Only 150 couples that underwent ICSI for male factor infertility agreed to participate, and 94 of them had retrieved enough FF samples suitable for laboratory testing. The FF sample was obtained, centrifuged, and stored in liquid nitrogen. Two organochlorine pesticides (OCPs), Lindane and DDT;three organophosphates (OPs);chlorpyrifos;Diazinon; malathion; one Chloroacetanilide (Pretilachlor);two pyrethroids(Bioallethrin and β-cyfluthrin); and the concentrations of four PCBs, 28, 52, 138, 180, were estimated in the obtained FF samples by using gas chromatography/mass spectrometry. SPSS statistical analysis program (version 17) was used for analysis. Multiple regression analysis was used to correlate the PCBs and pesticides with ICSI outcomes. Results There were significant negative correlations between FF concentrations of the eight examined pesticides and the four PCBs on the endometrial thickness. However, Pretilachlor, chlorpyrifos, β-cyfluthrin, and Diazinon were the only toxic agents that negatively correlated with the number of the oocytes retrieved. Fertilization and early embryo cleavage rates were negatively correlated with Pretilachlor and β-cyfluthrin. Moreover, high concentrations of Lindane,DDT, Diazinon,and chlorpyrifos were significantly associated lower implantation rate. PCB 28 and 180 concentration in the FF was associated with a lower number of retrieved oocytes and fertilization rate, respectively. The number of implanted embryos was negatively correlated with PCB 52 FF concentration. However, the clinical pregnancy rate did not reach the level of significance. Conclusion Higher concentrations of any studied PCBs and pesticides are associated with thinner endometrial thickness. The higher the level of Pretilachlor, β-cyfluthrin, PCB 28 and 180, the lower the retrieval, fertilization, and embryo cleavage rates. High PCB and pesticide concentrations in the FF adversely affected embryological ICSI outcomes. However, more data are needed to evaluate their effect on the clinical outcome. |
| Databáze: | OpenAIRE |
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