Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging
| Autor: | Vakoc, B. J., Lanning, R. M., Tyrrell, J. A., Padera, T. P., Bartlett, L. A., Stylianopoulos, T., Munn, L. L., Tearney, G. J., Fukumura, D., Jain, R. K., Bouma, Brett E. |
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| Přispěvatelé: | Stylianopoulos, T. [0000-0002-3093-1696] |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Pathology
Time Factors optical frequency domaing imaging animal cell Imaging angiogenesis Mice Optical frequencies Neoplasms Microscopy diphtheria toxin breast carcinoma Fluorescein Angiography Heterologous drug cytotoxicity three dimensional imaging article Lymphography General Medicine Equipment Design Domain imaging Lymphangiogenesis priority journal histopathology microscopy contrast enhancement Female diagnostic value Intravital microscopy cancer tissue Diagnostic Imaging medicine.medical_specialty Transplantation Heterologous lymphatic system Biology Article General Biochemistry Genetics and Molecular Biology animal tissue monoclonal antibody DC101 Experimental Imaging Three-Dimensional male tumor vascularization In vivo image analysis medicine Humans Animals controlled study human outcome assessment mouse tissue structure Tumor microenvironment Transplantation nonhuman human cell animal model Mammary Neoplasms Mammary Neoplasms Experimental treatment response Image Enhancement microenvironment image processing Microvessels Three-Dimensional Biophysics Glioblastoma |
| Zdroj: | Nature medicine |
| Popis: | Intravital multiphoton microscopy has provided powerful mechanistic insights into health and disease and has become a common instrument in the modern biological laboratory. The requisite high numerical aperture and exogenous contrast agents that enable multiphoton microscopy, however, limit the ability to investigate substantial tissue volumes or to probe dynamic changes repeatedly over prolonged periods. Here we introduce optical frequency domain imaging (OFDI) as an intravital microscopy that circumvents the technical limitations of multiphoton microscopy and, as a result, provides unprecedented access to previously unexplored, crucial aspects of tissue biology. Using unique OFDI-based approaches and entirely intrinsic mechanisms of contrast, we present rapid and repeated measurements of tumor angiogenesis, lymphangiogenesis, tissue viability and both vascular and cellular responses to therapy, thereby demonstrating the potential of OFDI to facilitate the exploration of physiological and pathological processes and the evaluation of treatment strategies. © 2009 Nature America, Inc. All rights reserved. 15 1219 1223 1219-1223 |
| Databáze: | OpenAIRE |
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