Identification of thyroid stimulating hormone receptor-specific T cells in Graves' disease thyroid using autoantigen-transfected Epstein-Barr virus-transformed B cell lines
| Autor: | S. B. A. Cohen, Marc Feldmann, Yuti Chernajovsky, R. J. Mullins, Marco Londei, Colin M. Dayan, Louise M. C. Webb |
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| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
endocrine system
Herpesvirus 4 Human endocrine system diseases medicine.medical_treatment Graves' disease T-Lymphocytes Antigen presentation Thyroid Gland Transfection Autoantigens Thyrotropin receptor Cell Line Antigen Thyroid peroxidase medicine Humans B cell Cell Line Transformed Antigen Presentation B-Lymphocytes biology business.industry Thyroid Receptors Thyrotropin General Medicine medicine.disease Virology Molecular biology Graves Disease medicine.anatomical_structure biology.protein Cytokines Thyroglobulin business Research Article |
| Popis: | The importance of thyrotropin receptor (TSHR) agonist antibodies in the manifestations of Graves' disease (GD) is recognized. There are, however, no convincing reports of TSHR-specific T cells. We have previously cloned T cells specific for thyroglobulin and thyroid peroxidase (TPO) from GD lymphoid infiltrates and used autologous EBV-transformed B cell lines (EBVL) transfected with an expression vector encoding TPO to efficiently detect TPO-specific T cells. Here we used EBVL transfected with TSHR to seek TSHR-specific T cells in the GD infiltrates, after cloning the in vivo activated T cells without antigen. 3 out of 30 clones responded vigorously and reproducibly to EBVL-TSHR, with a mean stimulation index > 7. Their release of IL-2, IL-4, and IL-10 after stimulation with soluble anti-CD3 and phorbol ester was indistinguishable from the other clones from this thyroid. However, they produced relatively little IFN gamma (median IL-4/IFN gamma ratio of 0.80) compared with the other clones (median IL-4/IFN gamma ratio 0.06). Thus, this new potent method of antigen presentation, using autoantigen-transfected EBVL, has permitted the first unequivocal identification of TSHR T cells in GD thyroid, with distinct Th0/Th2 characteristics, unlike previously cloned TPO-responsive cells which have Th1 characteristics. |
| Databáze: | OpenAIRE |
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