Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale
| Autor: | Hans J. Tanke, Lucia Birner, Tero Soukka, Antonín Hlaváček, Andreas Sedlmeier, Verena Muhr, Thomas Hirsch, Paul L. A. M. Corstjens, Hans H. Gorris, Matthias Jürgen Mickert |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
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Luminescence Laser scanning Nanoparticle 02 engineering and technology 010402 general chemistry 01 natural sciences Collimated light Analytical Chemistry law.invention Optics law Animals Glycoproteins Photons business.industry Chemistry Lasers ta1182 Helminth Proteins 021001 nanoscience & nanotechnology Laser Fluorescence Photon upconversion 0104 chemical sciences Antigens Helminth Nanoparticles Schistosoma 0210 nano-technology business |
| Zdroj: | Analytical Chemistry, 88(3), 1835-1841 |
| ISSN: | 0003-2700 |
| DOI: | 10.1021/acs.analchem.5b04147 |
| Popis: | An upconversion laser scanner has been optimized to exploit the advantages of photon-upconverting nanoparticles (UCNPs) for background-free imaging on a macroscopic scale. A collimated 980 nm laser beam afforded high local excitation densities to account for the nonlinear luminescence response of UCNPs. As few as 2000 nanoparticles were detectable, and the linear dynamic range covered more than 5 orders of magnitude, which is essentially impossible by using conventional fluorescent dyes. UCNPs covered by a dye-doped silica shell were separated by agarose gel electrophoresis and scanned by a conventional fluorescence scanner as well as the upconversion scanner. Both optical labels could be detected independently. Finally, upconversion images of lateral flow test strips were recorded to facilitate the sensitive and quantitative detection of disease markers. A marker for the parasitic worm Schistosoma was used in this study. |
| Databáze: | OpenAIRE |
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