β-defensin 2 as an Adjuvant Promotes Anti-Melanoma Immune Responses and Inhibits the Growth of Implanted Murine Melanoma In Vivo
| Autor: | Juan Shen, Xiao-bo Li, Xiaobao Jin, Qiang Wu, Ai-hua Zeng, Jiayong Zhu, Han-fang Mei, Xue-mei Lu |
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| Rok vydání: | 2012 |
| Předmět: |
beta-Defensins
T-Lymphocytes medicine.medical_treatment T cell Immunology Cancer Treatment Melanoma Experimental lcsh:Medicine chemical and pharmacologic phenomena Biology Cancer Vaccines Mice Immune system Adjuvants Immunologic Immunity medicine Animals Cytotoxic T cell lcsh:Science neoplasms Cell Proliferation Multidisciplinary Innate immune system lcsh:R hemic and immune systems Immunotherapy Immunity Innate respiratory tract diseases Killer Cells Natural medicine.anatomical_structure Beta defensin Oncology Medicine lcsh:Q biological phenomena cell phenomena and immunity Neoplasm Transplantation CD8 Research Article |
| Zdroj: | PLoS ONE, Vol 7, Iss 2, p e31328 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0031328 |
| Popis: | β-defensin 2 is a small antimicrobial peptide of the innate immune system and has been thought to regulate anti-tumor immunity. However, little is known on whether β-defensin 2 could modulate melanoma-specific NK and T cell responses. In this study, we first cloned the murine β-defensin 2 gene by RT-PCR and generated the β-defensin 2 stably expressing B16 cells (B16-mBD2). Subsequently, we evaluated whether vaccination with irradiated B16-mBD2 could modulate the growth of implanted B16 cells and determined the potential mechanisms underlying the action of B16-mBD2 vaccine in modulating the growth of B16 tumors in C57BL/6. We found that vaccination with irradiated B16-mBD2, but not with control B16-p or parental B16, inhibited the development and progression of B16 tumors, and prolonged the survival of tumor-bearing mice. However, vaccination with irradiated B16-mBD2 failed to inhibit the development of B16 tumors in the CD4(+)- or CD8(+)-depleted recipients. Furthermore, vaccination with irradiated B16-mBD2 stimulated strong NK activity and promoted potent B16-specific CTL responses, accompanied by augmenting IFN-γ and IL-12, but not IL-4, responses in the recipient mice. Moreover, vaccination with irradiated B16-mBD2 promoted the infiltration of CD8(+) and CD4(+) T, NK cells and macrophages in the tumor tissues. These data suggest β-defensin 2 may act as a positive regulator, promoting anti-tumor NK and T cell responses in vivo. Therefore, β-defensin 2 may be used for the development of immunotherapy for the intervention of melanoma. |
| Databáze: | OpenAIRE |
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