Cytotoxicity control of silicon nanoparticles by biopolymer coating and ultrasound irradiation for cancer theranostic applications
| Autor: | Alain Géloën, Tatiana S. Perova, Yu I Bezsudnova, Liubov A. Osminkina, A Yu Kharin, J V Kargina, M. B. Gongalsky, V. Yu. Timoshenko, Vladimir Lysenko, A.P. Sviridov, A. A. Kudryavtsev |
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| Přispěvatelé: | Department of Physiology, University of São Paulo (USP), International Laboratory BioNanophoton, National Research Nuclear University, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), InstituteTheoretical and Experiment Biophysics, the Russian Academy of Sciences [Moscow, Russia] (RAS), Department of Electrical and Electronic Engineering, Okayama University, ITMO Univiversity, Centre National de la Recherche Scientifique (CNRS), INL - Spectroscopies et Nanomatériaux (INL - S&N), Institut des Nanotechnologies de Lyon (INL), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Lyon (ECL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Russian Science Foundation 16-13-10145, 'Andre Mazon' program, INSA de Lyon, Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Russian Academy of Sciences, Okayama University [Okayama], Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École Centrale de Lyon (ECL), Université de Lyon, Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Luminescence Time Factors theranostic [SDV]Life Sciences [q-bio] mild cancer therapy Nanoparticle silicon si (symbol) sonosensitizers 02 engineering and technology Spectrum Analysis Raman ultrason nanoparticule Theranostic Nanomedicine Mice chemistry.chemical_compound silicium Coated Materials Biocompatible Neoplasms General Materials Science Cytotoxicity cellular proliferation Cell Death irradiation ultrasound nanoparticle cytotoxicité Dextrans 021001 nanoscience & nanotechnology Dextran Ultrasonic Waves Mechanics of Materials photoluminescence 0210 nano-technology Silicon Materials science Photoluminescence silicon nanoparticle Cell Survival chemistry.chemical_element Bioengineering Nanotechnology engineering.material Porous silicon 03 medical and health sciences Dogs 3T3-L1 Cells Cell Line Tumor ultrasonic treatment Animals Humans cancer Electrical and Electronic Engineering prolifération cellulaire Mechanical Engineering Sonodynamic therapy technology industry and agriculture General Chemistry 030104 developmental biology cell proliferation chemistry engineering Nanoparticles Biopolymer |
| Zdroj: | Nanotechnology Nanotechnology, Institute of Physics, 2017, 28 (10), pp.105102. ⟨10.1088/1361-6528/aa5b7c⟩ |
| ISSN: | 0957-4484 1361-6528 |
| DOI: | 10.1088/1361-6528/aa5b7c⟩ |
| Popis: | Silicon nanoparticles (SiNPs) prepared by mechanical grinding of luminescent porous silicon were coated with a biopolymer (dextran) and investigated as a potential theranostic agent for bioimaging and sonodynamic therapy. Transmission electron microscopy, photoluminescence and Raman scattering measurements of dextran-coated SiNPs gave evidence of their enhanced stability in water. In vitro experiments confirmed the. lower cytotoxicity of the dextran-coated NPs. in comparison with uncoated ones, especially for. high concentrations of about 2 mg ml(-1). Efficient uptake of the NPs. by cancer cells was found using bioimaging in the optical transmittance and photoluminescence modes. Treatment. of the cells with uptaken SiNPs by therapeutic ultrasound for 5-20 min resulted in a strong decrease in. the number of. living cells, while the total number of cells remained nearly unchanged. The obtained data indicate a ` mild' effect of the combined action of ultrasonic irradiation. and SiNPs on cancer cells. The observed results reveal new opportunities for. controlling the photoluminescent and sonosensitizing properties of silicon-based NPs. for applications in the diagnostics and mild therapy of cancer. |
| Databáze: | OpenAIRE |
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