Effect of Homogeneity on Cell Growth and Anthocyanin Biosynthesis in Suspension Cultures of Vitis vinifera
| Autor: | Xingju Yu, Wei Zhang, Jun-Ge Qu, Meifang Jin |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Methyl jasmonate
Light Cell growth fungi food and beverages Phenylalanine Secondary metabolite Biology Elicitor Anthocyanins chemistry.chemical_compound Laboratory flask Suspensions chemistry Cell culture Anthocyanin Botany medicine General Earth and Planetary Sciences Vitis Biomass Food science Cell Proliferation General Environmental Science medicine.drug |
| Zdroj: | Chinese Journal of Biotechnology. 22:805-810 |
| ISSN: | 1872-2075 |
| DOI: | 10.1016/s1872-2075(06)60057-5 |
| Popis: | The instability of secondary metabolite production is a ubiquitous problem in plant cell culture. To understand the instability, the investigation of anthocyanin accumulation in suspension cultures of Vitis vinifera, as a model system, has been initiated in our laboratory. Suspension culture of a relatively homogeneous cell line E of V. vinifera, was established by long-term cell line selection by anthocyanin content differentiation. The aggregate size of E was smaller than that of other cell lines obtained by routine screening method. The variation coefficients of anthocyanin content in suspension cultures of E were 8.7% in long-term subcultures and 5% in repeated flasks, respectively. The effects of elicitor, precursor feeding and light irridiation on biomass and anthocyanin accumulation in suspension cultures of E had been investigated and the results showed that all the variation coefficients were lower than 12% and this indicated the importance of homogeneity on stable production in plant cell culture. With the combination treatment of 30micromol/L phenylalanine and 218micromol/L methyl jasmonate in the dark in suspension cultures of E, the anthocyanin content and production in suspension culture of E was 5.89-fold and 4.30-fold of the controls, respectively, and all the variation coefficients of biomass and anthocyanin accumulation were lower than those of the controls in 5 successive subcultures. |
| Databáze: | OpenAIRE |
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