Effects of Synergistic Inhibition on α-glucosidase by Phytoalexins in Soybeans
| Autor: | Kiwon Lee, Chi-Yeol Yoo, Kyungjin Kim, Sang-Han Lee, Chang Hyung Lee, Myung-Ae Bae, Hogyun Seo, Chul-Hong Park, Eun-Kyeong Yoon, Hyeong-U Son, Tae Ho Kim |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
phytoalexins glyceollin Pterocarpans α-glucosidase inhibitor Genistein Biochemistry Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Gene Expression Regulation Plant enzyme kinetics medicine Glycoside Hydrolase Inhibitors Enzyme kinetics luteolin Molecular Biology Glyceollin Plant Proteins Chemistry fungi Daidzein food and beverages Combination index Drug Synergism alpha-Glucosidases Isoflavones 030104 developmental biology Mechanism of action Polyphenol combination index 030220 oncology & carcinogenesis Soybeans medicine.symptom daidzein Luteolin Sesquiterpenes |
| Zdroj: | Biomolecules Volume 9 Issue 12 |
| ISSN: | 2218-273X |
| Popis: | To determine the mechanism of action of the effects of phytoalexins in soybeans, we analyzed &alpha glucosidase inhibition kinetics using Michaelis&ndash Menten plots and Lineweaver&ndash Burk plots. The results showed that the type of inhibition with glyceollin was competitive, that of genistein was noncompetitive, that of daidzein was uncompetitive, and luteolin showed a mixed mode of action. The Ki values were determined using a Dixon plot as glyceollin, 18.99 &mu M genistein, 15.42 &mu luteolin, 16.81 &mu and daidzein, 9.99 &mu M. Furthermore, potential synergistic effects between glyceollin and the three polyphenols were investigated. A combination of glyceollin and luteolin at a ratio of 3:7 exhibited synergistic effects on &alpha glucosidase inhibition, having a combination index (CI) of 0.64244, according to the CI&ndash isobologram equation. Collectively, these results showed that a combination of glyceollin and luteolin has the potential to inhibit &alpha glucosidase activity via a synergistic mode of inhibition. |
| Databáze: | OpenAIRE |
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