Magnetic Rolling Circle Amplification-Assisted Single-Particle Collision Immunosensor for Ultrasensitive Detection of Cardiac Troponin I
| Autor: | Jie Yang, Juan He, Long Mi, Feng Han, Wei Wen, Xiuhua Zhang, Shengfu Wang, Zhen Wu |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Analytical Chemistry. 94:12514-12522 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.2c02774 |
| Popis: | Owing to its simplicity, high throughput, and ultrasensitivity, single-particle collision electrochemistry (SPCE) has attracted great attention in biosensing, especially labeled SPCE. However, the low signal conversion efficiency and much interference from complex samples limit its wide application. Here, a new and robust SPCE immunosensor was proposed for ultrasensitive cardiac troponin I (cTnI) detection by combining target-driven rolling circle amplification (RCA) with magnetic beads (MBs). Antibody-modified MBs have good stability, dispersity, and magnetic response capacity in complex samples, enabling efficient capture and separation of cTnI with high specificity and anti-interference ability. The presence of cTnI could specifically drive the formation of magnetic immunocomplexes followed by triggering RCA and enzyme digestion reaction. By using Pt nanoparticles (Pt NPs)-modified ssDNA as signal probes, one cTnI molecule could induce the release of 4.5 × 10 |
| Databáze: | OpenAIRE |
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