Expression of endothelial nitric oxide synthase in human peritoneal tissue: Regulation by Escherichia coli lipopolysaccharide
| Autor: | Juan C. de la Pinta, Lourdes Sánchez de Miguel, Octavio Frieyro, A. Reyero, Antonio López-Farré, María M. Arriero, José Fortes, F. González-Fernández, Juan A. Rodríguez-Feo, Angeles Franco, Santos Casado, Carlos Pastor, Angel Celdrán |
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| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Endothelium Nitric Oxide Synthase Type III Nitric oxide chemistry.chemical_compound Cytosol Enos medicine Escherichia coli Humans RNA Messenger 3' Untranslated Regions Messenger RNA biology Tumor Necrosis Factor-alpha Proteins General Medicine MRNA stabilization biology.organism_classification Molecular biology Mesothelium medicine.anatomical_structure chemistry Biochemistry Nephrology Immunologic Techniques Nitric Oxide Synthase Peritoneum |
| Zdroj: | Scopus-Elsevier ResearcherID |
| Popis: | Changes in the expression of endothelial nitric oxide synthase (eNOS) in the peritoneum could be involved in the peritoneal dysfunction associated with peritoneal inflamma- tion. Demonstrated recently in bovine endothelial cells was the existence of cytosolic proteins that bind to the 39-untranslated region (39-UTR) of eNOS mRNA and could be implicated in eNOS mRNA stabilization. The present work demonstrates that eNOS protein is expressed in human endothelial and mesothelial peritoneal cells. Escherichia coli lipopolysaccha- ride shortened the half-life of eNOS message, reducing eNOS protein expression in peritoneal mesothelial and endothelial cells. Moreover, under basal conditions, human peritoneal samples expressed cytosolic proteins that bind to the 39-UTR of eNOS mRNA. The cytosolic proteins that directly bind to 39-UTR were identified as a 60-kD protein. After incubation of human peritoneal samples with lipopolysaccharide, the binding activity of the cytosolic 60-kD protein increased in a time- dependent manner. Studies are now necessary to determine the involvement of this 60-kD protein in the regulation of eNOS expression in peritoneal cells and particularly its involvement in the peritoneal dysfunction associated with inflammatory reactions. |
| Databáze: | OpenAIRE |
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