Identification of Potential Auxin-Responsive Small Signaling Peptides through a Peptidomics Approach in Arabidopsis thaliana
| Autor: | Yuan Xiao, Qiwen Liang, Weigui Luo, Yi Su, Langtao Xiao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Proteomics plant protoplast Arabidopsis Pharmaceutical Science Gene Expression Tandem mass spectrometry 01 natural sciences Article Analytical Chemistry lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry Plant Growth Regulators Auxin Plant Cells Drug Discovery Auxin biosynthesis Arabidopsis thaliana Physical and Theoretical Chemistry 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Indoleacetic Acids Arabidopsis Proteins Gene Expression Profiling Protoplasts Organic Chemistry Phosphate buffered saline fungi Wild type peptidomics food and beverages Protoplast biology.organism_classification Plants Genetically Modified Cell biology Plant Leaves chemistry Chemistry (miscellaneous) small signaling peptides Molecular Medicine Signal transduction auxin Oligopeptides 010606 plant biology & botany Signal Transduction |
| Zdroj: | Molecules Molecules, Vol 24, Iss 17, p 3146 (2019) Volume 24 Issue 17 |
| ISSN: | 1420-3049 |
| Popis: | Small signaling peptides (SSPs) are a class of short peptides playing critical roles in plant growth and development. SSPs are also involved in the phytohormone signaling pathway. However, identification of mature SSPs is still a technical challenge because of their extremely low concentrations in plant tissue and complicated interference by many other metabolites. Here, we report an optimized protocol to extract SSPs based on protoplast extraction and to analyze SSPs based on tandem mass spectrometry peptidomics. Using plant protoplasts as the material, soluble peptides were directly extracted into phosphate buffer. The interference of non-signaling peptides was significantly decreased. Moreover, we applied the protocol to identify potential SSPs in auxin treated wild type and auxin biosynthesis defective mutant yuc2yuc6. Over 100 potential SSPs showed a response to auxin in Arabidopsis thaliana. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |