Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cells
| Autor: | David J. Orlicky, J.G. Lieber, C.L. Morin, R.M. Evans |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Messenger RNA
receptor regulatory protein Immunoprecipitation lipid accumulation Adipose tissue dexamethasone Cell Biology QD415-436 Biology Molecular biology Biochemistry adipogenesis Blot chemistry.chemical_compound prostaglandin F2α Endocrinology chemistry 3T3-L1 adipocytes Adipogenesis Adipocyte Lipid droplet Receptor |
| Zdroj: | Journal of Lipid Research, Vol 39, Iss 6, Pp 1152-1161 (1998) |
| ISSN: | 0022-2275 |
| Popis: | Synthesis and accumulation of the recently iden- tified prostaglandin F 2 a receptor regulatory protein (FPRP) was found to correlate closely with lipid droplet accumula- tion by 3T3-L1 preadipose cells. FPRP, a transmembrane glycoprotein, has been shown to regulate the binding of ligand to certain seven-transmembrane receptors. Anti- FPRP immunohistochemistry, Western blotting, and meta- bolic labeling/immunoprecipitation experiments demon- strated that FPRP was not detectable in undifferentiated 3T3-L1 cells. Interestingly, low levels of FPRP mRNA were detected in the undifferentiated 3T3-L1 cells. After induc- tion of adipose differentiation, FPRP mRNA increased , 3 fold whereas FPRP synthesis increased , 50 fold. Differen- tiation induction with either dexamethasone/insulin/isobu- tylmethylxanthine or the thiazolidinedione derivative ADD 4743 were both effective at inducing FPRP accumulation and accumulation of lipid droplets. By co-immunohisto- chemical and lipid staining, greater than 99% of the cells ac- cumulating lipid droplets possessed FPRP. FPRP mRNA and protein are also found in rat adipose tissue. Treatment of 3T3-L1 cells with an FPRP anti-sense oligonucleotide during differentiation decreased FPRP accumulation and resulted in a decrease in lipid droplets without altering the level of induction of a late marker of adipocyte differentia- tion, glycerol-3-phosphate dehydrogenase activity. Transient expression of an FPRP cDNA in undifferentiated 3T3-L1 cells was insufficient to induce lipid droplet accumula- tion.— Orlicky, D. J., J. G. Lieber, C. L. Morin, and R. M. Evans. Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3- L1 cells. J. Lipid Res. 1998. 39: 1152-1161. |
| Databáze: | OpenAIRE |
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