Drosophila YBX1 homolog YPS promotes ovarian germ line stem cell development by preferentially recognizing 5-methylcytosine RNAs
| Autor: | Fan Zou, Shiyuan Chen, Ting Xie, Renjun Tu, Allison R. Scott, Sisi Li, Hua Li, Xiaoqing Song, Zhenlin Yang, Andrew Price, Anoja Perera, Zhaohua Ping, Bo Duan |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Multidisciplinary RNA methylation 030302 biochemistry & molecular biology RNA Biological Sciences Y box binding protein 1 Biology Germline Cell biology 03 medical and health sciences 5-Methylcytosine chemistry.chemical_compound chemistry Stem cell Function (biology) 030304 developmental biology Adult stem cell |
| Zdroj: | Proc Natl Acad Sci U S A |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | 5-Methylcytosine (m 5 C) is a RNA modification that exists in tRNAs and rRNAs and was recently found in mRNAs. Although it has been suggested to regulate diverse biological functions, whether m 5 C RNA modification influences adult stem cell development remains undetermined. In this study, we show that Ypsilon schachtel (YPS), a homolog of human Y box binding protein 1 (YBX1), promotes germ line stem cell (GSC) maintenance, proliferation, and differentiation in the Drosophila ovary by preferentially binding to m 5 C-containing RNAs. YPS is genetically demonstrated to function intrinsically for GSC maintenance, proliferation, and progeny differentiation in the Drosophila ovary, and human YBX1 can functionally replace YPS to support normal GSC development. Highly conserved cold-shock domains (CSDs) of YPS and YBX1 preferentially bind to m 5 C RNA in vitro. Moreover, YPS also preferentially binds to m 5 C-containing RNAs, including mRNAs, in germ cells. The crystal structure of the YBX1 CSD-RNA complex reveals that both hydrophobic stacking and hydrogen bonds are critical for m 5 C binding. Overexpression of RNA-binding–defective YPS and YBX1 proteins disrupts GSC development. Taken together, our findings show that m 5 C RNA modification plays an important role in adult stem cell development. |
| Databáze: | OpenAIRE |
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