Treatment of giant congenital melanocytic nevi with cultured epithelial autografts: Clinical and histopathological analysis
| Autor: | Hiroki Yamanaka, Naoki Morimoto, Jun Arata, Itaru Tsuge, Shuji Ogino, Motoki Katsube, Michiharu Sakamoto, A. Shoji-Pietraszkiewicz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty CEA cultured epithelial autograft Medicine (General) GCMN giant congenital melanocytic nevi medicine.medical_treatment Abrasion (medical) Biomedical Engineering Curettage Dermal regeneration Biomaterials 03 medical and health sciences Hypertrophic scar 0302 clinical medicine R5-920 Dermis CMN congenital melanocytic nevi Medicine Nevus Giant congenital melanocytic nevus medicine.diagnostic_test integumentary system QH573-671 business.industry Dermabrasion medicine.disease Surgery Cultured epidermis 030104 developmental biology medicine.anatomical_structure Skin biopsy Original Article Epidermis business Cytology 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Regenerative Therapy, Vol 18, Iss, Pp 1-6 (2021) Regenerative Therapy |
| ISSN: | 2352-3204 |
| Popis: | Introduction Curettage and dermabrasion are effective in the treatment of giant congenital melanocytic nevi (GCMN); however, local infection and hypertrophic scar formation are major issues. Thus, we applied cultured epithelial autografts (CEA) on skin defects after curettage or abrasion of GCMN and assessed the postoperative outcomes. Methods Seven nevi lesions of five patients (aged 3 months to 24 years) were treated with CEA after curettage or abrasion with a dermatome or a surgical bar, respectively. We assessed the postoperative outcomes, including CEA take ratio, erosion and/or ulcer formation in the acute phase, hospitalization days, Vancouver scar scale, and color improvement one year after the operation. In addition, a histological evaluation of a skin biopsy was performed over one year after the operation. Results The CEAs took well on the wound, and the wound surface was mostly epithelized by postoperative day 7 in all cases. While hypertrophic scar formation and slight pigmentation were observed in some lesions, the color was improved in all of the treated lesions. Histopathological examination revealed that the regenerated epidermis had stratified keratinocytes with rete ridges, and the dermal layer without nevus cells regenerated above the remaining dermis layer. Conclusions In this study, we found that early epithelialization and regeneration of the dermal layer was achieved after the application of CEA, suggesting that CEA could be an effective option after curettage or abrasion of GCMN. Highlights • Cultured epithelial autograft (CEA) application is effective in the treatment of giant congenital melanocytic nevi. • The grafted CEA takes well on skin defects post curettage and abrasion. • CEAs can achieve rapid epithelization. • Normal dermal tissue regenerates after CEA application. |
| Databáze: | OpenAIRE |
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