Nanostructured lipid carriers containing chitosan or sodium alginate for co-encapsulation of antioxidants and an antimicrobial agent for potential application in wound healing

Autor: Marinilce Fagundes dos Santos, Gabriel Lima Barros de Araujo, Marlus Chorilli, Jenyffer Kelly Rocha De Matos, Luciana B. Lopes, Giovanna Cassone Salata, Sandra Costa-Fernandez, Kelly Ishida, Il-Sei Watanabe, Gaby S. Scheunemann
Přispěvatelé: Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp)
Rok vydání: 2021
Předmět:
FOLHAS (PLANTAS)
Antioxidant
medicine.medical_treatment
alpha-Tocopherol
Argan oil
Chick Embryo
02 engineering and technology
Biochemistry
Antioxidants
Chitosan
chemistry.chemical_compound
Anti-Infective Agents
Cell Movement
Structural Biology
Zeta potential
Food science
Cells
Cultured
Skin
Drug Carriers
0303 health sciences
Chemistry
Tea tree oil
General Medicine
021001 nanoscience & nanotechnology
Antimicrobial
Lipids
Pseudomonas aeruginosa
Quercetin
0210 nano-technology
medicine.drug
Staphylococcus aureus
food.ingredient
Alginates
Drug Compounding
Bioadhesive
Sodium
Wound healing
chemistry.chemical_element
03 medical and health sciences
food
Tea Tree Oil
medicine
Animals
Humans
Plant Oils
Molecular Biology
030304 developmental biology
Sapotaceae
Wound Healing
Bioadhesion
Nanostructured lipid carriers
Fibroblasts
Nanoparticles
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 0141-8130
Popis: Made available in DSpace on 2021-06-25T11:15:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-07-31 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The high incidence and costs of chronic wounds in the elderly have motivated the search for innovations to improve product performance and the healing process while reducing costs. In this study, bioadhesive nanostructured lipid carriers (NLC) were developed for the co-encapsulation of compounds with antioxidant (α-tocopherol and quercetin) and antimicrobial (tea tree oil) activity for management of wounds. The NLC was produced with shea butter and argan oil, and modified with sodium alginate or chitosan to confer bioadhesive properties. Spherical nanoparticles of ~307–330 nm and zeta potential varying from −21.2 to +11.8 mV were obtained. Thermal analysis demonstrated that the lipid matrix reduced tea tree oil thermal loss (~1.8-fold). Regardless of the type of polysaccharide employed, the NLCs promoted cutaneous localization of antioxidants in damaged (subjected to incision) skin, with a ~74 to 180-fold higher delivery into the skin compared to percutaneous delivery. This result is consistent with the similar bioadhesive properties of chitosan or sodium alginate-modified NLC. Nanoencapsulation of tea tree oil did not preclude its antimicrobial effects against susceptible and resistant strains of S. aureus and P. aeruginosa, while co-encapsulation of antioxidants increased the NLC-induced fibroblasts migration, supporting their potential usefulness for management of wounds. Instituto de Ciências Biomédicas Universidade de São Paulo School of Pharmaceutical Sciences São Paulo State University (UNESP) Faculdade de Ciências Farmacêuticas Universidade de São Paulo School of Pharmaceutical Sciences São Paulo State University (UNESP) CAPES: 001 FAPESP: 2013/16617-7 FAPESP: 2015/07993-0 FAPESP: 2017/19059-6 FAPESP: 2017/19374-9 FAPESP: 2018/13877-1 FAPESP: 2019/26048-6 CNPq: 306866/2020-0
Databáze: OpenAIRE
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