Caveolin-1 is important for nitric oxide-mediated angiogenesis in fibrin gels with human umbilical vein endothelial cells
| Autor: | Zhang-hua Zhu, Yu-Dong Qiu, Xitai Sun, Yiming Pan, Yongzhong Yao, Yitao Ding |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Vascular Endothelial Growth Factor A
Umbilical Veins Nitric Oxide Synthase Type III Angiogenesis Caveolin 1 Neovascularization Physiologic Fibrin Umbilical vein Nitric oxide chemistry.chemical_compound Downregulation and upregulation Enos Medicine Humans Pharmacology (medical) RNA Messenger Cells Cultured Pharmacology biology business.industry Endothelial Cells General Medicine Oligonucleotides Antisense biology.organism_classification Molecular biology Vascular endothelial growth factor NG-Nitroarginine Methyl Ester chemistry Immunology cardiovascular system biology.protein business |
| Zdroj: | Acta pharmacologica Sinica. 27(12) |
| ISSN: | 1671-4083 |
| Popis: | Aim: The role of caveolin-1 (Cav-1) in angiogenesis remains poorly understood. The endothelial nitric oxide (NO) synthase (eNOS), a caveolin-interacting protein, was demonstrated to play a predominant role in vascular endothelial growth factor (VEGF) -induced angiogenesis. The purpose of our study was to examine the role of Cav-1 and the eNOS complex in NO-mediated angiogenesis. Methods: Human umbilical vein endothelial cells (HUVEC) were isolated and cultured in 3-D fibrin gels to form capillary-like tubules by VEGF stimulation. The expression of Cav-1 and eNOS was detected by semiquantitative RT-PCR. The HUVEC were treated with antisense oligonucleotides to downregulate Cav-1 expression. Both transduced and non-infected HUVEC were cultured in fibrin gels in the presence or absence of VEGF (20 ng/mL) and N G -nitro- L -arginine methyl ester ( L -NAME; 5 mmol/L). NO was measured using a NO assay kit and capillary-like tubules were quantified by tubule formation index using the Image J program. Results: RT-PCR analysis revealed that Cav-1 levels steadily increased in a time-dependent manner and reached their maximum after 5 d of incubation, but there were no obvious changes in eNOS mRNA expression in response to VEGF in the fibrin gel model. VEGF (20 ng/mL) can promote NO production and the formation of capillary-like tubules, and this promoting effect of VEGF was blocked by the addition of L-NAME (5 mmol/L). When transduced HUVEC with the antisense Cav-1 oligonucleotides were plated in the fibrin gels, the capillary-like tubules were significantly fewer than those of the non-infected cells. The capillary-like tubules formation and NO production of transduced HUVEC with the antisense Cav-1 oligonucleotides cultured in fibrin gels showed no responses to the addition of VEGF (20 ng/mL) and L-NAME (5.0 mmol/L). Conclusion: NO was a critical angiogenic mediator in this model. Cav-1 was essential for NO-mediated angiogenesis and may be an important target of anti-angiogenesis therapy. |
| Databáze: | OpenAIRE |
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