Intrathecal Transplantation of Autologous and Allogeneic Bone Marrow-Derived Mesenchymal Stem Cells in Dogs

Autor: Diana Milena Rodríguez Hurtado, Gisele Fabrino Machado, Marta Cristina Thomas Heckler, Guilherme D. Melo, Felipe Pérez Benavides, L. R. Teixeira, Rogério Martins Amorim, Giovana Boff Araujo Pinto, Marina Mitie Monobe, Diego Noé Rodríguez-Sánchez, Fernanda da Cruz Landim e Alvarenga
Přispěvatelé: Lyssavirus, épidémiologie et neuropathologie - Lyssavirus Epidemiology and Neuropathology, Institut Pasteur [Paris], Universidade Estadual Paulista (UNESP)
Rok vydání: 2021
Předmět:
Pathology
medicine.medical_specialty
matrix metalloproteinase
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
cell-based therapy
Cell
Central nervous system
Biomedical Engineering
Cell- and Tissue-Based Therapy
Matrix metalloproteinase
Intrathecal
Mesenchymal Stem Cell Transplantation
Transplantation
Autologous
cerebrospinal fluid
03 medical and health sciences
0302 clinical medicine
Cerebrospinal fluid
Dogs
Bone Marrow
medicine
Animals
Transplantation
Homologous
Injections
Spinal
030304 developmental biology
0303 health sciences
Transplantation
medicine.diagnostic_test
business.industry
intrathecal route
Mesenchymal stem cell
bone marrow mesenchymal stem cells
Magnetic resonance imaging
Mesenchymal Stem Cells
Cell Biology
3. Good health
medicine.anatomical_structure
Medicine
Original Article
business
030217 neurology & neurosurgery
Zdroj: Cell Transplantation
Cell Transplantation, Cognizant Communication Corporation, 2021, 30, pp.096368972110344. ⟨10.1177/09636897211034464⟩
Cell Transplantation, Vol 30 (2021)
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1555-3892
0963-6897
DOI: 10.1177/09636897211034464⟩
Popis: Made available in DSpace on 2022-04-29T08:32:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 The route used in the transplantation of mesenchymal stem cells (MSCs) can directly affect the treatment success. The transplantation of MSCs via the intrathecal (IT) route can be an important therapeutic strategy for neurological disorders. The objective of this study was to evaluate the safety and feasibility of the IT transplantation of autologous (Auto-MSCs) and allogeneic (Allo-MSCs) bone marrow mesenchymal stem cells (BM-MSCs) in healthy dogs. Based on neurodisability score, cerebrospinal fluid (CSF) and magnetic resonance imaging (MRI), no significant differences from the control group were observed on day 1 or day 5 after IT Auto- or Allo-MSCs transplantation (P > 0.05). In addition, analysis of matrix metalloproteinase (MMP)-2 and MMP-9 expression in the CSF revealed no significant differences (P > 0.05) at 5 days after IT transplantation in the Auto- or Allo-MSCs group when compared to the control. Intrathecal transplantation of BM-MSCs in dogs provides a safe, easy and minimally invasive route for the use of cell-based therapeutics in central nervous system diseases. Department of Veterinary Clinics School of Veterinary Medicine and Animal Science São Paulo State University (UNESP) Department of Animal Reproduction and Veterinary Radiology São Paulo State University (UNESP) Department of Veterinary Surgery and Anesthesiology São Paulo State University (UNESP) Department of Clinics Surgery and Animal Reproduction São Paulo State University (UNESP) Department of Veterinary Clinics School of Veterinary Medicine and Animal Science São Paulo State University (UNESP) Department of Animal Reproduction and Veterinary Radiology São Paulo State University (UNESP) Department of Veterinary Surgery and Anesthesiology São Paulo State University (UNESP) Department of Clinics Surgery and Animal Reproduction São Paulo State University (UNESP)
Databáze: OpenAIRE
Externí odkaz: