Light history influences the response of the marine cyanobacterium Synechococcus sp. WH7803 to oxidative stress
| Autor: | Nicolas Blot, Douglas A. Campbell, Gildas Le Corguillé, Annabelle Monnier, Anne Peyrat, Laurence Garczarek, Christophe Six, Morgane Ratin, Daniella Mella-Flores, Priscillia Gourvil, Christophe Boutte |
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| Přispěvatelé: | Procaryotes Phototrophes Marins = MArine Phototrophic Prokaryotes (MAPP), Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Photosynthetic Molecular Ecophysiology (Biology Department), Mount Allison University, MArine Phototrophic Prokaryotes (MAPP), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Centre National de la Recherche Scientifique (CNRS)-Université d'Auvergne - Clermont-Ferrand I (UdA), Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Photoinhibition
Light Photosystem II Physiology Acclimatization Plant Science MESH: Base Sequence medicine.disease_cause Photochemistry MESH: Paraquat Cluster Analysis Photosynthesis MESH: Bacterial Proteins MESH: Photosynthesis Oligonucleotide Array Sequence Analysis Synechococcus chemistry.chemical_classification 0303 health sciences MESH: Gene Expression Regulation Bacterial MESH: Oxidative Stress MESH: Photosystem II Protein Complex Cell biology MESH: Synechococcus MESH: Regulon MESH: Hydrogen Peroxide Repressor lexA MESH: Genes Bacterial Paraquat Molecular Sequence Data MESH: Acclimatization Environmental Stress and Adaptation to Stress Biology Regulon MESH: Multivariate Analysis Electron Transport 03 medical and health sciences Bacterial Proteins Genetics medicine Seawater MESH: Electron Transport 030304 developmental biology Reactive oxygen species MESH: Molecular Sequence Data Base Sequence 030306 microbiology MESH: Transcriptome Photosystem II Protein Complex MESH: Seawater Gene Expression Regulation Bacterial Hydrogen Peroxide biology.organism_classification MESH: Cluster Analysis [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Light Oxidative Stress chemistry Genes Bacterial Photoprotection Multivariate Analysis MESH: Oligonucleotide Array Sequence Analysis [SDE.BE]Environmental Sciences/Biodiversity and Ecology Transcriptome Oxidative stress |
| Zdroj: | Plant Physiology Plant Physiology, 2011, 156 (4), pp.1934-54. ⟨10.1104/pp.111.174714⟩ Plant Physiology, American Society of Plant Biologists, 2011, 156 (4), pp.1934-54. ⟨10.1104/pp.111.174714⟩ |
| ISSN: | 0032-0889 1532-2548 |
| DOI: | 10.1104/pp.111.174714⟩ |
| Popis: | Marine Synechococcus undergo a wide range of environmental stressors, especially high and variable irradiance, which may induce oxidative stress through the generation of reactive oxygen species (ROS). While light and ROS could act synergistically on the impairment of photosynthesis, inducing photodamage and inhibiting photosystem II repair, acclimation to high irradiance is also thought to confer resistance to other stressors. To identify the respective roles of light and ROS in the photoinhibition process and detect a possible light-driven tolerance to oxidative stress, we compared the photophysiological and transcriptomic responses of Synechococcus sp. WH7803 acclimated to low light (LL) or high light (HL) to oxidative stress, induced by hydrogen peroxide (H2O2) or methylviologen. While photosynthetic activity was much more affected in HL than in LL cells, only HL cells were able to recover growth and photosynthesis after the addition of 25 μm H2O2. Depending upon light conditions and H2O2 concentration, the latter oxidizing agent induced photosystem II inactivation through both direct damage to the reaction centers and inhibition of its repair cycle. Although the global transcriptome response appeared similar in LL and HL cells, some processes were specifically induced in HL cells that seemingly helped them withstand oxidative stress, including enhancement of photoprotection and ROS detoxification, repair of ROS-driven damage, and regulation of redox state. Detection of putative LexA binding sites allowed the identification of the putative LexA regulon, which was down-regulated in HL compared with LL cells but up-regulated by oxidative stress under both growth irradiances. |
| Databáze: | OpenAIRE |
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