Chronic stimulation induces adaptive potassium channel activity that restores calcium oscillations in pancreatic islets in vitro
| Autor: | Isabella Marinelli, Kathryn L. Corbin, Cara Schildmeyer, Nathan C. Law, Richard Bertram, Craig S. Nunemaker |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Potassium Channels Physiology Endocrinology Diabetes and Metabolism Stimulation Congenital hyperinsulinemia Calcium in biology Potassium Chloride Mice 0302 clinical medicine KATP Channels Insulin-Secreting Cells Glucose homeostasis Chemistry Depolarization Adaptation Physiological Stimulation Chemical Potassium channel activity medicine.anatomical_structure Islets Research Article medicine.drug medicine.medical_specialty Oscillations Tolbutamide β-cells 030209 endocrinology & metabolism Islets of Langerhans 03 medical and health sciences Physiology (medical) Internal medicine Potassium Channel Blockers medicine Animals Humans Channel blocker Calcium Signaling Potassium Channels Inwardly Rectifying Intracellular calcium Persistent hyperinsulinemic hypoglycemia of infancy Pancreatic islets Cell Membrane Models Theoretical Pulsatility 030104 developmental biology Endocrinology PHHI Potassium Congenital Hyperinsulinism K(ATP)-channel |
| Zdroj: | Am J Physiol Endocrinol Metab |
| ISSN: | 1522-1555 0193-1849 |
| DOI: | 10.1152/ajpendo.00482.2019 |
| Popis: | Insulin pulsatility is important to hepatic response in regulating blood glucose. Growing evidence suggests that insulin-secreting pancreatic β-cells can adapt to chronic disruptions of pulsatility to rescue this physiologically important behavior. We determined the time scale for adaptation and examined potential ion channels underlying it. We induced the adaptation both by chronic application of the ATP-sensitive K+ [K(ATP)] channel blocker tolbutamide and by application of the depolarizing agent potassium chloride (KCl). Acute application of tolbutamide without pretreatment results in elevated Ca2+ as measured by fura-2AM and the loss of endogenous pulsatility. We show that after chronic exposure to tolbutamide (12–24 h), Ca2+ oscillations occur with subsequent acute tolbutamide application. The same experiment was conducted with potassium chloride (KCl) to directly depolarize the β-cells. Once again, following chronic exposure to the cell stimulator, the islets produced Ca2+ oscillations when subsequently exposed to tolbutamide. These experiments suggest that it is the chronic stimulation, and not tolbutamide desensitization, that is responsible for the adaptation that rescues oscillatory β-cell activity. This compensatory response also causes islet glucose sensitivity to shift rightward following chronic tolbutamide treatment. Mathematical modeling shows that a small increase in the number of K(ATP) channels in the membrane is one adaptation mechanism that is compatible with the data. To examine other compensatory mechanisms, pharmacological studies provide support that Kir2.1 and TEA-sensitive channels play some role. Overall, this investigation demonstrates β-cell adaptability to overstimulation, which is likely an important mechanism for maintaining glucose homeostasis in the face of chronic stimulation. |
| Databáze: | OpenAIRE |
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