Lissajous-like patterns in scatter plots of calibration beads
| Autor: | Peter M. A. Sloot, Jan Greve, Alfons G. Hoekstra, B.G. de Grooth, R.M.P. Doornbos, K.E.I. Deurloo |
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| Přispěvatelé: | Computational Science Lab (IVI, FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
Materials science
Lorenz-Mie theory Biophysics Multiangle light scattering Molecular physics Light scattering Pathology and Forensic Medicine symbols.namesake Endocrinology Polarization Analytical light scattering Rayleigh scattering Instrumentation Alignment Scattering Cell Biology Hematology Flow Cytometry Small particle light scattering Mueller matrix Calibration Light scattering by particles symbols Scattering theory Biological small-angle scattering Flow cytometer |
| Zdroj: | Cytometry, 16(3), 236-242. Wiley Cytometry, 16, 236-242. John Wiley and Sons Inc. |
| ISSN: | 0196-4763 |
| DOI: | 10.1002/cyto.990160307 |
| Popis: | Flow cytometric measurements of light scattering of polystyrene calibration beads revealed remarkable Lissajous-like loops in two-parameter scatter plots. The existence of such loops is shown to be in qualitative agreement with Lorenz-Mie scattering theory of homogeneous spheres. The occurrence of these patterns reflects the extreme particle size dependency of perpendicular light scattering of homogeneous spheres. These effects may give an explanation for the frequently observed phenomenon that polystyrene spheres reveal a relatively large CV in the perpendicular light scattering signals, whereas the CV of the forward light scattering signal is small. We conclude that one should be careful to optimize the perpendicular light scattering channel by minimizing the CV, because there is no linear relationship between instrument alignment quality and the CV of the perpendicular light scattering signals of calibration beads. |
| Databáze: | OpenAIRE |
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