The vacuole controls nucleolar dynamics and micronucleophagy via the NVJ
| Autor: | Most Naoshia Tasnin, Takashi Ushimaru, Tasnuva Sharmin, Shamsul Morshed, Tsuneyuki Takuma |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Biophysics Autophagy-Related Proteins Receptors Cytoplasmic and Nuclear Saccharomyces cerevisiae Vacuole Mechanistic Target of Rapamycin Complex 1 DNA Ribosomal Biochemistry 03 medical and health sciences 0302 clinical medicine Protein purification medicine Receptor Molecular Biology Ribosomal DNA Cell Nucleus Microbial Viability Chemistry Dynamics (mechanics) Nuclear Proteins Cell Biology Nutrient starvation Cell biology 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Vacuoles RDNA condensation Nucleus Cell Nucleolus Protein Binding |
| Zdroj: | Biochemical and Biophysical Research Communications. 550:158-165 |
| ISSN: | 0006-291X |
| Popis: | Chromosomes have their own territories and dynamically translocate in response to internal and external cues. However, whether and how territories and the relocation of chromosomes are controlled by other intracellular organelles remains unknown. Upon nutrient starvation and target of rapamycin complex 1 (TORC1) inactivation, micronucleophagy, which preferentially degrades nucleolar proteins, occurs at the nucleus-vacuole junction (NVJ) in budding yeast. Ribosomal DNA (rDNA) is condensed and relocated against the NVJ, whereas nucleolar proteins move towards the NVJ for micronucleophagic degradation, causing dissociation of nucleolar proteins from rDNA. These findings imply that the NVJ is the critical platform in the directional movements of rDNA and nucleolar proteins. Here, we show that cells lacking the NVJ (NVJΔ cells) largely lost rDNA condensation and rDNA-nucleolar protein separation after TORC1 inactivation. The macronucleophagy receptor Atg39, an outer nuclear membrane protein, accumulated at the NVJ and was degraded by micronucleophagy. These suggested that macronucleophagy is also dependent on the presence of the NVJ. However, micronucleophagy, but not macronucleophagy, was abolished in NVJΔ cells. This study clearly demonstrated that vacuoles controls intranuclear events, nucleolar dynamics, from outside of the nucleus via the NVJ under the control of TORC1. |
| Databáze: | OpenAIRE |
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