MazF activation causes ACA sequence-independent and selective alterations in RNA levels in Escherichia coli
| Autor: | Yoshio Takata, Yuya Netsu, Yuya Nakamura, Kazuki Fujisawa, Koji Nishikawa, Shotaro Ayukawa, Nobutaka Nakashima, Nobuhiro Hayashi, Hiro Kondo, Yoh-ichi Tagawa, Masayuki Yamamura, Kentaro Akiyama, Yuta Kino |
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| Rok vydání: | 2019 |
| Předmět: |
RNA-Seq
Biology medicine.disease_cause Biochemistry Microbiology Transcriptome 03 medical and health sciences Downregulation and upregulation Endoribonucleases Genetics medicine Escherichia coli Molecular Biology Gene 030304 developmental biology 0303 health sciences Escherichia coli K12 030306 microbiology Escherichia coli Proteins Chromosome RNA General Medicine Gene Expression Regulation Bacterial Cell biology DNA-Binding Proteins Horizontal gene transfer |
| Zdroj: | Archives of microbiology. 202(1) |
| ISSN: | 1432-072X |
| Popis: | Escherichia coli MazF is a toxin protein that cleaves RNA at ACA sequences. Its activation has been thought to cause growth inhibition, primarily through indiscriminate cleavage of RNA. To investigate responses following MazF activation, transcriptomic profiles of mazF-overexpressing and non-overexpressing E. coli K12 cells were compared. Analyses of differentially expressed genes demonstrated that the presence and the number of ACA trimers in RNA was unrelated to cellular RNA levels. Mapping differentially expressed genes onto the chromosome identified two chromosomal segments in which upregulated genes formed clusters, and these segments were absent in the chromosomes of E. coli strains other than K12. These results suggest that MazF regulates selective, rather than indiscriminate, categories of genes, and is involved in the regulation of horizontally acquired genes. We conclude that the primary role of MazF is not only cleaving RNA indiscriminately but also generating a specific cellular state. |
| Databáze: | OpenAIRE |
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