Reduced eosinophil apoptosis in induced sputum correlates with asthma severity
| Autor: | Garry Michael Walsh, Morgan Graeme Blaylock, J. G. Douglas, A. Lawrie, Christopher J A Duncan |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Spirometry Sputum Cytology Apoptosis Severity of Illness Index Allergic inflammation Leukocyte Count Random Allocation Severity of illness Humans Medicine Aged Asthma medicine.diagnostic_test business.industry Sputum Middle Aged respiratory system Airway obstruction Eosinophil medicine.disease respiratory tract diseases Eosinophils Cross-Sectional Studies medicine.anatomical_structure Case-Control Studies Chronic Disease Immunology Female medicine.symptom business |
| Zdroj: | European Respiratory Journal. 22:484-490 |
| ISSN: | 1399-3003 0903-1936 |
| DOI: | 10.1183/09031936.03.00109803a |
| Popis: | This study was carried out to investigate the relationship between induced sputum eosinophil apoptosis and clinical severity score, airway obstruction and symptom scores in patients with chronic stable asthma. Altogether, 41 chronic stable asthmatic subjects of varying severity defined by Aas score and 17 control subjects underwent spirometry, symptom questionnaire and successful sputum induction. Sputum was processed and cytospins prepared for light microscopy to determine normal and apoptotic eosinophils. Mild asthmatic subjects had a significantly lower percentage sputum eosinophils and a significantly higher eosinophil apoptotic ratio (AR) than moderate or chronic severe asthmatics. Severe asthmatic subjects had a significantly greater age, duration of asthma and sputum eosinophil count x mL(-1) than mild asthmatic subjects. Asthmatic subjects' symptom scores, severity scores and age inversely correlated with AR and the percentage of sputum eosinophils. Baseline forced expiratory volume in one second inversely correlated with percentage sputum eosinophils and positively correlated with AR. The study demonstrates a relationship between reduced sputum eosinophil apoptosis and increased clinical severity of chronic stable asthma, providing additional evidence that eosinophil apoptosis may be important in the resolution of eosinophilic airway inflammation in asthma. |
| Databáze: | OpenAIRE |
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