Single cell analysis of nutrient regulation of Clostridioides (Clostridium) difficile motility
| Autor: | Melissa Madrill, Alden J. Rinehold, Astha Pokhrel, David S. Courson, Erin B. Purcell, Richard E. Cheney, Rita Tamayo, Cody Scott |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Intravital Microscopy biology Clostridioides difficile 030306 microbiology Cell Motility Nutrients Clostridium difficile biology.organism_classification Microbiology Article Cell biology 03 medical and health sciences Infectious Diseases Nutrient Clostridium medicine.anatomical_structure Single-cell analysis Live cell imaging medicine Anaerobic bacteria Locomotion 030304 developmental biology |
| Zdroj: | Anaerobe |
| ISSN: | 1075-9964 |
| DOI: | 10.1016/j.anaerobe.2019.102080 |
| Popis: | Regulation of bacterial motility to maximize nutrient acquisition or minimize exposure to harmful substances plays an important role in microbial proliferation and host colonization. The technical difficulties of performing high-resolution live microscopy on anaerobes have hindered mechanistic studies of motility in Clostridioides (formerly Clostridium) difficile. Here, we present a widely applicable protocol for live cell imaging of anaerobic bacteria that has allowed us to characterize C. difficile swimming at the single-cell level. This accessible method for anaerobic live cell microscopy enables inquiry into previously inaccessible aspects of C. difficile physiology and behavior. We present the first report that vegetative C. difficile are capable of regulated motility in the presence of different nutrients. We demonstrate that the epidemic C. difficile strain R20291 exhibits regulated motility in the presence of multiple nutrient sources by modulating its swimming velocity. This is a powerful illustration of the ability of single-cell studies to explain population-wide phenomena such as dispersal through the environment. |
| Databáze: | OpenAIRE |
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