Characterization of the Chemical Profile of Euphorbia Species from Turkey by Gas Chromatography-Mass Spectrometry (GC-MS), Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS), and Liquid Chromatography-Ion Trap-Time-of-Flight-Mass Spectrometry (LC-IT-TOF-MS) and Chemometric Analysis
| Autor: | Erhan Kaplaner, Mehmet Öztürk, Hamdi Temel, Mustafa Yilmaz, Pelin Köseoğlu Yılmaz, Ismail Yener, Abdulselam Ertas, Gulacti Topcu, Ufuk Kolak, Yeter Yeşil, Özge Tokul Ölmez |
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| Přispěvatelé: | MÜ, Fen Fakültesi, Kimya Bölümü, Ölmez, Özge Tokul, Öztürk, Mehmet |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Clinical Biochemistry
Gas Chromatography-Mass Spectrometry (GC-MS) 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry Euphorbia Liquid chromatography–mass spectrometry Euphorbia Liquid Chromatography-Ion Trap-Time-Of-Flight-Mass Spectrometry (LC-IT-TOF-MS) Lc ms ms Electrochemistry Spectroscopy Principal Component Analysis (PCA) Chromatography Chemistry 010401 analytical chemistry Biochemistry (medical) Chemometric Approach 021001 nanoscience & nanotechnology 0104 chemical sciences Hierarchical Cluster Analysis (HCA) Method Validation Ion trap Time-of-flight mass spectrometry Gas chromatography–mass spectrometry 0210 nano-technology Liquid Chromatography-Tandem Mass Spectrometry (LC-M MS) |
| Popis: | 0000-0001-8932-4535; 0000-0002-2093-4949; WOS: 000461872800001 The Euphorbiaceae family comprises of about 300 genera and 5000 species primarily distributed in America and tropical Africa. The Euphorbia genus is represented by 105 species and locally named as "Sutlegen" and "Xasil" in Turkey. The present study aimed to determine the chemical constituents of E. aleppica, E. eriophora, E. macroclada, E. grisophylla, E. seguieriana subsp. seguieriana, E. craspedia, E. denticulata, E. falcata, and E. fistulosa, and classify them by utilizing the chemometric techniques of principal component analysis (PCA) and hierarchical cluster analysis (HCA). Linoleic acid, 17-tetratriacontane, palmitic acid, and hexatriacontane were the major fatty acids from the gas chromatography-mass spectrometry (GC/MS) analyses. Characterization of 268 constituents of the studied species was achieved by liquid chromatography-ion trap-time-of-flight-mass spectrometry (LC-IT-TOF-MS). Furthermore, a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous quantitative determination of 11 compounds (quinic acid, protocatechuic acid, rutin, hesperidin, eugenol, p-coumaric acid, piceatannol, scopoletin, dl-kavain, chrysophanic acid, and resiniferatoxin) in these species. The developed method was validated for the linearity, limit of detection, limit of quantification, repeatability, and recovery. Mugla Sitki Kocman University Research FundMugla Sitki Kocman University [15/22] The Mugla Sitki Kocman University Research Fund is also acknowledged for the GC-MS analyses of the samples (Project number: 15/22). |
| Databáze: | OpenAIRE |
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