Supplementary Materials and Methods, Supplementary Table 2, and Supplementary Figures 1 and 2 from Neutralization of KIT Oncogenic Signaling in Leukemia with Antibodies Targeting KIT Membrane Proximal Domain 5

Autor: Marie-Alix Poul, André Pèlegrin, James D. Marks, Yu Zhou, Yongfeng Fan, Christian Auclair, Madeline Neiveyans, Ronan Crépin, Marianne Le Gall
Rok vydání: 2023
Popis: Materials and methods describing the production of scFv-Fc in HEK-T cells and KIT domains and scFv display on yeast. Table S2: Primers for PCR amplification of KIT and cloning into pYD vector by Gap repair. Figure S1: Selection and initial characterization of a panel of anti-KIT scFv. Figure S2: Anti-KIT D5 epitope characterization using yeast display.
Databáze: OpenAIRE