Melatonin and glycine reduce uterus ischemia/reperfusion injury in a rat model of warm ischemia

Autor: Mindaugas Kvietkauskas, Bettina Leber, Viktorija Zitkute, Peter Schemmer, Diana Ramasauskaite, Kęstutis Strupas, Philipp Stiegler, Vygante Maskoliunaite
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Uterus
melatonin
Antioxidants
Rats
Sprague-Dawley
0302 clinical medicine
Warm Ischemia
Biology (General)
Spectroscopy
biology
Glycine Agents
General Medicine
Computer Science Applications
Chemistry
medicine.anatomical_structure
030220 oncology & carcinogenesis
Myeloperoxidase
Reperfusion Injury
Female
medicine.drug
medicine.medical_specialty
QH301-705.5
Ischemia
Glycine
Catalysis
Article
Inorganic Chemistry
Melatonin
03 medical and health sciences
Internal medicine
Uterus transplantation
medicine
Animals
cardiovascular diseases
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
Cell damage
business.industry
urogenital system
Organic Chemistry
medicine.disease
ischemia and reperfusion injury
Rats
Disease Models
Animal
030104 developmental biology
Endocrinology
biology.protein
business
uterus transplantation
Reperfusion injury
Zdroj: International journal of molecular sciences, Basel : MDPI, 2021, vol. 22, art. no. 8373, p. [1-12]
International Journal of Molecular Sciences
Volume 22
Issue 16
International Journal of Molecular Sciences, Vol 22, Iss 8373, p 8373 (2021)
ISSN: 1661-6596
1422-0067
Popis: Ischemia/reperfusion injury (IRI) remains a significant problem to be solved in uterus transplantation (UTx). Melatonin and glycine have been shown to possess direct cytoprotective activities, mainly due to their antioxidative and anti-inflammatory properties. The aim of this study was to investigate the protective effects of melatonin and glycine and their combination on IRI in a rat model of warm ischemia. In this study, Sprague-Dawley rats were assigned to eight groups, including sham and IRI (n = 80). Melatonin and glycine alone or their combination were administered prior to 1 h of uterus ischemia followed by 1 h of reperfusion. Melatonin (50 mg/kg) was administered via gavage 2 h before IRI and glycine in an enriched diet for 5 days prior to intervention. Uterus IRI was estimated by histology, including immunohistochemistry, and biochemical tissue analyses. Histology revealed that uterus IRI was significantly attenuated by pretreatment with melatonin (p = 0.019) and glycine (p = 0.044) alone as well as their combination (p = 0.003). Uterus IRI led to increased myeloperoxidase expression, which was significantly reduced by melatonin (p = 0.004), glycine (p <
0.001) or their combination (p <
0.001). The decline in superoxide dismutase activity was significantly reduced in the melatonin (p = 0.027), glycine (p = 0.038) and combined treatment groups (p = 0.015) when compared to the IRI control group. In conclusion, melatonin, glycine and their combination significantly reduced oxidative stress-induced cell damage after IRI in a small animal warm ischemia model, and, therefore, clinical studies are required to evaluate the protective effects of these well-characterized substances in uterus IRI.
Databáze: OpenAIRE
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