Selective neurotoxic lesions of descending serotonergic and noradrenergic pathways in the rat
| Autor: | Lars Tveiten, Ole Bernt Fasmer, Odd-Geir Berge, Kjell Hole |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Male
medicine.medical_specialty Serotonin Central nervous system 5 7-Dihydroxytryptamine Biology Serotonergic Biochemistry Cellular and Molecular Neuroscience Norepinephrine chemistry.chemical_compound Hydroxydopamines Internal medicine Neural Pathways medicine Neurotoxin Animals Dihydroxytryptamines Oxidopamine Dose-Response Relationship Drug Rats Inbred Strains Spinal cord Frontal Lobe Rats Lumbar Spinal Cord Endocrinology medicine.anatomical_structure chemistry Spinal Cord Anesthesia 5 6-Dihydroxytryptamine medicine.drug Brain Stem Synaptosomes |
| Zdroj: | Journal of neurochemistry. 44(4) |
| ISSN: | 0022-3042 |
| Popis: | The ability of neurotoxic substances to induce selective lesions of the descending monoaminergic pathways in rats was investigated. Saline, 6-hydroxydopamine, 5,6-dihydroxytryptamine, or 5,7-dihydroxytryptamine were administered into the lumbar subarachnoid space through a chronically indwelling catheter. The lesions were evaluated 2-3 weeks later by in vitro uptake of [3H]noradrenaline and [14C]5-hydroxytryptamine into synaptosomal preparations from the frontal cortex, brainstem, cervical spinal cord, and lumbar spinal cord of each animal. There was no difference in uptake between saline-injected and noncatheterized controls and no significant changes in cortical uptake after any of the treatments (dose range of neurotoxins: 0.6-80 micrograms). In the lumbar spinal cord, 6-hydroxydopamine (5-80 micrograms) reduced the [3H]noradrenaline uptake by approximately 90% with no effects on [14C]5-hydroxytryptamine uptake, whereas 5,6-dihydroxytryptamine reduced the uptake of [14C]5-hydroxytryptamine by 90% (20-80 micrograms). [3H]Noradrenaline uptake was unaffected by lower doses of 5,6-dihydroxytryptamine but fell by 45-55% after 40-80 micrograms. 5,7-Dihydroxytryptamine (10-80 micrograms) reduced [3H]noradrenaline uptake by 90-95% and [14C]5-hydroxytryptamine uptake by approximately 80% (5-80 micrograms) in the lumbar cord. It is concluded that intrathecal administration of suitable doses of neurotoxins may produce extensive selective lesions of descending noradrenergic and serotonergic pathways. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text