Shiga toxin genes in avian Escherichia coli
Autor: | Carlton L. Gyles, Valeria R. Parreira |
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Rok vydání: | 2002 |
Předmět: |
DNA
Bacterial Turkeys animal structures Shigella dysenteriae Sequence analysis Shiga Toxin 1 medicine.disease_cause Polymerase Chain Reaction Shiga Toxin 2 Microbiology law.invention chemistry.chemical_compound fluids and secretions Shiga-like toxin Neutralization Tests law Pathogenic Escherichia coli STX2 Chlorocebus aethiops Escherichia coli medicine Animals Vero Cells Escherichia coli Infections Poultry Diseases Polymerase chain reaction General Veterinary biology Nucleic Acid Hybridization O Antigens Shiga toxin Sequence Analysis DNA General Medicine biology.organism_classification Virology chemistry biology.protein Chickens |
Zdroj: | Veterinary Microbiology. 87:341-352 |
ISSN: | 0378-1135 |
Popis: | The purpose of this study was to determine the presence of stx genes in avian pathogenic Escherichia coli (APEC). We examined 97 APEC isolates: 34 from lesions of avian cellulitis, 31 from avian septicemia, 13 from swollen head syndrome (SHS) in chickens, and 19 from diseased turkeys. We also examined five isolates from the feces of healthy chickens. All 102 E. coli isolates were tested for the presence of stx genes by PCR amplification and by colony blots using probes specific for stx1 and stx2. Fifty-three percent (52) of the 97 APEC carried stx gene sequences: one isolate carried stx2 sequences, two carried both stx1 and stx2 sequences, and the remaining 49 isolates carried only stx1 sequences. Twenty-six isolates were positive by both hybridization and PCR amplification, 10 were positive by PCR only, and 16 were positive by hybridization only. All the stx-positive isolates were negative by PCR for the eae and E-hlyA genes. The five isolates from healthy chickens were all negative for stx. All 13 SHS isolates were positive for the stx1 gene and had low titres for cytotoxicity in the Vero cell assay (VCA). Other stx-positive isolates were negative in the VCA. The stx1 gene from one SHS E. coli isolate was cloned and sequenced and shown to be identical to that of the stx gene of Shigella dysenteriae. The observations indicate that stx1 gene sequences are widespread among APEC but that cytotoxicity on Vero cells is uncommon. |
Databáze: | OpenAIRE |
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