The short consensus repeats 1 and 2, not the cytoplasmic domain, of human CD46 are crucial for infection of subgroup B adenovirus serotype 35
| Autor: | Akira Yamamoto, Naoki Okada, Tsukasa Seya, Takao Hayakawa, Hiroyuki Mizuguchi, Sayaka Murakami, Fuminori Sakurai, Kenji Kawabata |
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| Rok vydání: | 2006 |
| Předmět: |
viruses
Transgene Mutant Pharmaceutical Science CHO Cells Biology Transfection Membrane Cofactor Protein Transduction (genetics) Cricetulus Genes Reporter Transduction Genetic Cricetinae Animals Binding site Luciferases Receptor Genetics Binding Sites CD46 Adenoviruses Human Antibodies Monoclonal Virology female genital diseases and pregnancy complications Protein Structure Tertiary Mutation biology.protein Receptors Virus Antibody |
| Zdroj: | Journal of Controlled Release. 113:271-278 |
| ISSN: | 0168-3659 |
| DOI: | 10.1016/j.jconrel.2006.05.007 |
| Popis: | Human CD46 (membrane cofactor protein) has recently been identified to be an attachment receptor for subgroup B adenoviruses (Ads); however, the precise interaction between human CD46 and subgroup B Ads are just beginning to be understood. In this study, to characterize the interaction between human CD46 and subgroup B Ads, varieties of mutant CD46 were tested for their ability to act as a receptor for Ad serotype 35 (Ad35), which belongs to subgroup B. In addition, we determined Ad35 vector-mediated transgene expression and cellular uptake of Ad35 vectors in the presence of a set of anti-CD46 antibodies. Our data demonstrated that the short consensus repeats (SCRs) 1 and 2 in human CD46 are important for interaction with Ad35, whereas the cytoplasmic domain of human CD46 was found not to be required for the function as an Ad35 receptor. Rather, a complete deletion of the cytoplasmic domain of human CD46 increased the transduction efficiencies of Ad35 vectors. This information should help in elucidation of the mechanism of subgroup B Ad infection, as well in the improvement of the subgroup B Ad vectors. |
| Databáze: | OpenAIRE |
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