Urinalysis of atrazine exposure in farm pesticide applicators
| Autor: | David C. Christiani, John Mathew, Melissa J. Perry, John Strauss, James Tortorelli, David Degenhardt, William C. Sonzogni |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
0301 basic medicine
Urinalysis Health Toxicology and Mutagenesis Metabolite Enzyme-Linked Immunosorbent Assay Urine 010501 environmental sciences Toxicology 01 natural sciences Diluted urine Sensitivity and Specificity Gas Chromatography-Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound Occupational Exposure medicine Humans Atrazine 0105 earth and related environmental sciences Triazine Chromatography 030102 biochemistry & molecular biology medicine.diagnostic_test Chemistry Herbicides Public Health Environmental and Occupational Health Agriculture Pesticide Triazine herbicide Environmental chemistry Agrochemicals Environmental Monitoring |
| Zdroj: | Toxicology and industrial health. 16(7-8) |
| ISSN: | 0748-2337 |
| Popis: | This study compared three relatively common laboratory methods for the detection of atrazine (a triazine herbicide commonly used in US agriculture), and related metabolites in urine. Ninety-nine samples collected from atrazine applicators within 8 h post application were analyzed. Thirty-seven percent of applicators showed detectable levels (minimum=1.0 ng/ml) of deethylatrazine (an atrazine metabolite typically found in environmental samples) in their urine, based on gas chromatography-mass spectrometry (GC-MS) analysis (mean=14.2 ng/ml). Fifty applicator samples were tested using an enzyme-linked immunosorbent assay (ELISA) designed for the mercapturate metabolic product. Most of these samples (80%) had detectable levels of the mercapturate product. A triazine in water ELISAwas also used to test several diluted urine samples from atrazine applicators, and all samples were positive for triazines. Mediocre agreements between the three methods indicated that each detected distinct atrazine exposure products. The results indicate that single field applications of atrazine result in measurable pesticide doses to applicators and that the choice of field assay should depend on the exposure product to be evaluated. |
| Databáze: | OpenAIRE |
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