Molecular cloning, genomic structure, and tissue distribution of EW135, a novel chicken egg white protein with group B scavenger receptor cysteine-rich domains
| Autor: | Misao Matsushita, Hideki Asanuma, Whayoung Yoo, Tomohiro Nakamura |
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| Rok vydání: | 2013 |
| Předmět: |
Signal peptide
DNA Complementary Molecular Sequence Data Immunology Oviducts Biology Molecular cloning Polymerase Chain Reaction Tandem repeat Consensus Sequence Genetics Consensus sequence Animals Tissue Distribution Amino Acid Sequence Cysteine Cloning Molecular Gene Peptide sequence chemistry.chemical_classification Base Sequence Sequence Homology Amino Acid Egg Proteins Exons Genomics Scavenger Receptors Class B Molecular biology Introns Protein Structure Tertiary Amino acid chemistry Female Chickens Egg white |
| Zdroj: | Immunogenetics. 65:785-793 |
| ISSN: | 1432-1211 0093-7711 |
| DOI: | 10.1007/s00251-013-0723-8 |
| Popis: | Approximately 80 proteins are reported to be present in chicken egg white. The major function of egg white proteins isolated so far is to defend the egg yolk against infections. We recently isolated a novel protein termed EW135 from chicken egg white. In this paper, we have determined the complete amino acid sequence of EW135 based on cDNA cloning. EW135 consists of 970 amino acids with a putative signal peptide of 17 amino acids. It is composed exclusively of tandem repeats of nine group B scavenger receptor cysteine-rich (SRCR) domains separated by eight seven-amino acid peptides. The features of consensus sequences found in the group B SRCR domain were well conserved in EW135. The EW135 gene consists of putative 11 exons, with each SRCR domain being encoded by a single exon. Reverse transcription PCR showed that EW135 is expressed in only the oviduct among the 11 types of tissues tested. EW135 is a second soluble protein belonging to the group B SRCR domain superfamily identified in chickens. One of the important functions of proteins belonging to the group B SRCR domain superfamily is to recognize pathogens in innate immunity. It is, therefore, conceivable that EW135 could be involved in host defense in egg white. |
| Databáze: | OpenAIRE |
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