Comparison of Western immunoblot antigens and interpretive criteria for detection of antibody to human T-lymphotropic virus types I and II
| Autor: | D Gallo, J L Diggs, C V Hanson |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Microbiology (medical)
Radioimmunoprecipitation Assay Deltaretrovirus Antigens viruses Blotting Western Retroviridae Proteins Oncogenic Fluorescent Antibody Technique Human T-lymphotropic virus Virus Immunoenzyme Techniques Viral Envelope Proteins Western blot Antigen immune system diseases hemic and lymphatic diseases medicine Humans biology medicine.diagnostic_test Gene Products env virus diseases biology.organism_classification Virology Molecular biology Recombinant Proteins HTLV-I Antibodies HTLV-II Antibodies Evaluation Studies as Topic Immunoassay biology.protein Indicators and Reagents Antibody Research Article |
| Zdroj: | Journal of Clinical Microbiology. 28:2045-2050 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.28.9.2045-2050.1990 |
| Popis: | Twenty human T-lymphotropic virus type I (HTLV-I) antibody-positive sera from Japan, Hawaii, and the Marshall Islands and 15 HTLV type II (HTLV-II) antibody-positive sera from intravenous drug users in the United States were tested by immunoblotting with two recombinant HTLV-I proteins and three commercial kits to determine whether there were any differences in reactions between HTLV-I- and HTLV-II-positive sera by the Western immunoblot method and, also, to evaluate the ability of these reagents to detect HTLV-I- and HTLV-II-seropositive individuals by using the recommended Western blot interpretation. These sera were first extensively characterized by immunofluorescence, enzyme immunoassay, radioimmunoprecipitation assay, and Western blot using HTLV-I and HTLV-II viral lysates and an envelope (env) recombinant protein. Although both HTLV-I- and HTLV-II antibody-positive sera reacted with the env protein gp68, reactions with the gp46 env antigens appeared to be specific for HTLV-I. It was found that the use of either p19 or p24 core bands plus an env reaction instead of only the p24 plus env reaction (as presently recommended) increased the number of positive interpretations for HTLV-I but had no effect on the number of HTLV-II-positive interpretations. |
| Databáze: | OpenAIRE |
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