Nucleotide-Binding Leucine-Rich Repeat Genes CsRSF1 and CsRSF2 Are Positive Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea

Autor: Xue Wang, Huang Jingnan, Haiyan Fan, Na Cui, Yang Yu, Xiangnan Meng, Qiumin Chen
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0106 biological sciences
0301 basic medicine
NBS-LRR
01 natural sciences
Cucumis sativus
lcsh:Chemistry
chemistry.chemical_compound
Gene Expression Regulation
Plant
Plant defense against herbivory
Abscisic acid
lcsh:QH301-705.5
Spectroscopy
Sphaerotheca fuliginea
Disease Resistance
Plant Proteins
Methyl jasmonate
biology
food and beverages
General Medicine
Computer Science Applications
transient genetic transformation
Host-Pathogen Interactions
Gibberellin
Cotyledon
Cucumis
Subcellular Fractions
NLR Proteins
Genes
Plant
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Ascomycota
Gene Silencing
expression analysis
Physical and Theoretical Chemistry
Molecular Biology
Gene
Organic Chemistry
Subcellular localization
biology.organism_classification
Molecular biology
030104 developmental biology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Salicylic acid
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 3986, p 3986 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 8
ISSN: 1661-6596
1422-0067
Popis: Cucumber powdery mildew caused by Sphaerotheca fuliginea is a leaf disease that seriously affects cucumber’s yield and quality. This study aimed to report two nucleotide-binding site-leucine-rich repeats (NBS-LRR) genes CsRSF1 and CsRSF2, which participated in regulating the resistance of cucumber to S. fuliginea. The subcellular localization showed that the CsRSF1 protein was localized in the nucleus, cytoplasm, and cell membrane, while the CsRSF2 protein was localized in the cell membrane and cytoplasm. In addition, the transcript levels of CsRSF1 and CsRSF2 were different between resistant and susceptible cultivars after treatment with exogenous substances, such as abscisic acid (ABA), methyl jasmonate (MeJA), salicylic acid (SA), ethephon (ETH), gibberellin (GA) and hydrogen peroxide (H2O2). The expression analysis showed that the transcript levels of CsRSF1 and CsRSF2 were correlated with plant defense response against S. fuliginea. Moreover, the silencing of CsRSF1 and CsRSF2 impaired host resistance to S. fuliginea, but CsRSF1 and CsRSF2 overexpression improved resistance to S. fuliginea in cucumber. These results showed that CsRSF1 and CsRSF2 genes positively contributed to the resistance of cucumber to S. fuliginea. At the same time, CsRSF1 and CsRSF2 genes could also regulate the expression of defense-related genes. The findings of this study might help enhance the resistance of cucumber to S. fuliginea.
Databáze: OpenAIRE
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