Effects of Conditioned Medium of Co-Culture IL-2 Induced NK Cells and Human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) on Apoptotic Gene Expression in a Breast Cancer Cell Line (MCF-7)
| Autor: | Philips Onggowidjaja, Rizal Rizal, Diana Krisanti Jasaputra, Indra Bachtiar, Mochammad Aris Widodo, Ervi Afifah, Annisa Amalia, Harry Murti, Wahyu Widowati, Sutiman Bambang Sumitro, Dwi Davidson Rihibiha, Hanna Sari Widya Kusuma |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
General Mathematics Cell General Physics and Astronomy General Biochemistry Genetics and Molecular Biology 03 medical and health sciences breast cancer 0302 clinical medicine medicine Cytotoxic T cell lcsh:Science lcsh:Science (General) natural killer cells Multidisciplinary biology Chemistry Cell growth Mesenchymal stem cell General Chemistry General Medicine conditioned medium 030104 developmental biology medicine.anatomical_structure Perforin Granzyme MCF-7 Apoptosis 030220 oncology & carcinogenesis biology.protein Cancer research General Earth and Planetary Sciences human wharton’s jelly mesenchymal stem cells interleukin-2 lcsh:Q General Agricultural and Biological Sciences lcsh:Q1-390 |
| Zdroj: | Journal of Mathematical and Fundamental Sciences, Vol 51, Iss 3, Pp 205-224 (2019) |
| ISSN: | 2338-5510 2337-5760 |
| Popis: | Breast cancer (BC) is the most prevalent type of cancer among women and one of the major causes of cancer mortality in women. Metastasis in breast cancer (BC) occurs due to immunosurveillance deficiency, including impairment of natural killer (NK) cell maturation. Conditioned medium (CM) from human Wharton’s jelly mesenchymal stem cells (hWJMSC-CM) is known to possess anticancer activity. The CM of co-culture of human recombinant IL-2 treated NK cells and hWJMSCs is expected to boost anticancer activity toward BC cells which can be analyzed from the effect of CM towards secretion of effector molecules and expression of BC cell apoptosis-related genes, and cytotoxic granules in human recombinant IL-2 treated NK (IL-2 NK) and hWJMSCs (IL-2 hWJMSCs). TNF-α, IFN-γ, perforin, granzyme were measured by ELISA, while the inhibition of cell proliferation was measured by MTS assay and BC cell apoptosis by flow cytometry and apoptotic gene expression by RTPCR. CM from co-cultured hWJMSCs and IL-2 NK cells inhibited NK and BC cell proliferation, increased expression of Bax and p53 and decreased the number of Bcl-2 in BC cells. In conclusion, CM of co-culture IL-2 treated NK cells and hWJMSCs induce apoptosis in BC cells as indicated by increased Bax and p53 expression and decreased Bcl-2 expression. |
| Databáze: | OpenAIRE |
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